Veselkov A N, Davis D, Dymant L N, Parkes H, Shipp D
Sevastopol Instrument-Making Institute, Burkback College, London University, Great Britain.
Biofizika. 1992 Sep-Oct;37(5):851-8.
Temperature relationships of chemical shifts of protons of proflavin mixed with deoxytetraribonucleoside triphosphate 5'-d(ApGpCpT) in water solution were investigated on impulse NMR spectrometer (500 MHz). Procedure is suggested for calculating values of mole fractions of various associates in solution as a function against temperature. Free energies of Gibbs, entalpy and entropy were determined in the reactions of complex formation 1:1, 1:2, 2:1 of proflavin with tetranucleotide. The results point to a significant role of hydrophobic interactions during the formation of dye--tetramere duplex complexes.
在脉冲核磁共振波谱仪（500兆赫）上研究了在水溶液中，原黄素与脱氧四核糖核苷三磷酸5'-d（ApGpCpT）混合时质子化学位移的温度关系。提出了一种计算溶液中各种缔合物摩尔分数值随温度变化的方法。测定了原黄素与四核苷酸形成1:1、1:2、2:1配合物反应的吉布斯自由能、焓和熵。结果表明，疏水相互作用在染料-四聚体双链体配合物形成过程中起重要作用。
