Veselkov A N, Dévis D, Dymant L N, Parkes Kh
Biofizika. 1993 Jul-Aug;38(4):627-35.
Equilibrium behaviour of self-complimentary deoxytetranucleotides d(ApGpCpT) in D2O solution has been studied by the method of proton magnetic resonance spectroscopy (500 MHz). Complete assignments of the d(ApGpCpT) protons were obtained from both two-dimensional homonuclear COSY- and NOESY-experiments. Concentration and temperature dependences of tetranucleotide proton chemical shifts have been investigated. The equilibrium association constants and thermodynamical parameters of self-association of the molecules have been calculated using theoretical models and regression equations based on experimental results.
通过质子磁共振波谱法（500兆赫）研究了自互补脱氧四核苷酸d（ApGpCpT）在重水溶液中的平衡行为。通过二维同核COSY和NOESY实验完成了d（ApGpCpT）质子的完全归属。研究了四核苷酸质子化学位移的浓度和温度依赖性。利用基于实验结果的理论模型和回归方程计算了分子自缔合的平衡缔合常数和热力学参数。
