Teska J D, Cipriano R C, Schill W B
U.S. Fish and Wildlife Service, National Fish Health Research Laboratory, Kearneysville, West Virginia 25430.
J Wildl Dis. 1992 Oct;28(4):515-20. doi: 10.7589/0090-3558-28.4.515.
Cytochrome oxidase variants of the bacterial fish pathogen, Aeromonas salmonicida, were characterized for genetic and molecular homology with cytochrome oxidase-positive isolates that typically induce furunculosis in salmonids. Protein and lipopolysaccharide moieties of the cytochrome oxidase-negative variants were similar to their typical counterparts, based on sodium-dodecyl-sulfate polyacrylamide gel electrophoresis. Pathogenicity of aberrant isolates to brook trout (Salvelinus fontinalis) was similar to typical cytochrome oxidase-positive isolates. Colorimetric deoxyribonucleic acid (DNA) hybridization in 96-well microplates yielded homology values greater than 82.5% for typical aberrant A. salmonicida isolates when photobiotinylated DNA for reference A. salmonicida 3.101 was used as a probe. The only variation of these isolates from typical A. salmonicida was a negative cytochrome oxidase reaction.
对细菌性鱼类病原体杀鲑气单胞菌的细胞色素氧化酶变体进行了表征，以确定其与通常在鲑科鱼类中引发疖疮病的细胞色素氧化酶阳性分离株的遗传和分子同源性。基于十二烷基硫酸钠聚丙烯酰胺凝胶电泳，细胞色素氧化酶阴性变体的蛋白质和脂多糖部分与其典型对应物相似。异常分离株对溪红点鲑（Salvelinus fontinalis）的致病性与典型的细胞色素氧化酶阳性分离株相似。当使用光生物素化的杀鲑气单胞菌3.101 DNA作为探针时，在96孔微孔板中进行的比色脱氧核糖核酸（DNA）杂交显示，典型的异常杀鲑气单胞菌分离株的同源性值大于82.5%。这些分离株与典型杀鲑气单胞菌的唯一差异是细胞色素氧化酶反应呈阴性。
