Lardelli P, Manzano D, Steinberg S M, Madariaga L, Antón I, Cisterna R
Department of Microbiology and Immunology, School of Medicine, University of the Basque Country, Bilbao, Spain.
Microbiol Immunol. 1992;36(8):905-9. doi: 10.1111/j.1348-0421.1992.tb02093.x.
The existence of molecular transactivations between EBV and HIV-1, as well as reactivations of EBV latent infections in AIDS patients, have been recently documented. In order to shed more light on the putative association between EBV and HIV, and its role in the evolution to AIDS, we have determined simultaneously p24 protein and EBV DNA in culture supernatants of peripheral blood mononuclear cells from 47 individuals suspected of having HIV infection. The results of the in vitro assays were correlated with the clinical stage of the individuals and their serologic status to EBV. Statistical analysis showed a concordance between HIV infection and in vitro detection of EBV DNA (P < 0.002); particularly, a strong correlation between the presence of EBV DNA and p24 in culture was observed (P < 0.001). These results are consistent with the occurrence of viral interactions, manifested in vitro. However, in our series, the appearance of EBV DNA in culture was not concomitant with an elevation of anti-VCA IgG titers, anti-EA titers or the development of symptomatology, suggestive of a reactivation of a latent EBV infection or a progression of HIV infection. Therefore we conclude that, although interaction between both viruses may take place at the molecular level, there is no clear evidence of the repercussion that this event may have on the clinical course of HIV infection.
最近有文献记载了EB病毒（EBV）与人类免疫缺陷病毒1型（HIV-1）之间存在分子反式激活作用，以及艾滋病患者中EBV潜伏感染的再激活情况。为了更深入了解EBV与HIV之间可能存在的关联及其在艾滋病发展过程中的作用，我们同时检测了47名疑似感染HIV的个体外周血单个核细胞培养上清液中的p24蛋白和EBV DNA。体外检测结果与个体的临床分期及其针对EBV的血清学状态相关。统计分析表明，HIV感染与体外检测到EBV DNA之间存在一致性（P < 0.002）；特别是，在培养物中观察到EBV DNA的存在与p24之间存在强烈相关性（P < 0.001）。这些结果与体外表现出的病毒相互作用情况一致。然而，在我们的研究系列中，培养物中EBV DNA的出现并未伴随着抗VCA IgG滴度升高、抗EA滴度升高或症状出现，这表明潜伏的EBV感染再激活或HIV感染进展。因此我们得出结论，尽管两种病毒之间可能在分子水平发生相互作用，但没有明确证据表明这一事件可能对HIV感染的临床病程产生影响。
