Wehling P, Pak M, Cleveland S, Nieper R
Orthopädische Klinik, Heinrich-Heine-Universität Düsseldorf, Federal Republic of Germany.
Acta Neurochir (Wien). 1992;119(1-4):121-7. doi: 10.1007/BF01541795.
Regeneration of peripheral nerve fibers is impeded by the formation of scar tissue at the site of injury. The possible beneficial effect of collagenase on nerve regeneration was studied using clinical, neurophysiological (evoked potentials) and histological (nerve fiber counts) methods. The sciatic nerves of rats were transected and the severed ends abutted and sewn together. In one series, the area about the lesion was covered with fibrin adhesive and infused with either isotonic saline (controls) or collagenase (treatment group). In the other series, the severed ends of the nerve were inserted into a silicone tube and separated by a collagen plug, which was infused with either saline or collagenase. Compared to the controls, the treated animals showed a significant improvement of clinical and neurophysiological parameters. After 3 months of observation, the collagen content of the transection site was reduced, and in the silicone series, the total number of myelinated axons 5 mm distal to the site of transection was increased, while the fiber diameter distribution was unchanged.
损伤部位瘢痕组织的形成会阻碍周围神经纤维的再生。使用临床、神经生理学（诱发电位）和组织学（神经纤维计数）方法研究了胶原酶对神经再生可能的有益作用。切断大鼠坐骨神经，将断端对接并缝合。在一个系列中，用纤维蛋白粘合剂覆盖损伤部位，并注入等渗盐水（对照组）或胶原酶（治疗组）。在另一个系列中，将神经断端插入硅胶管中，并用胶原栓隔开，胶原栓注入盐水或胶原酶。与对照组相比，治疗组动物的临床和神经生理学参数有显著改善。观察3个月后，横断部位的胶原含量降低，在硅胶管系列中，横断部位远端5mm处有髓轴突的总数增加，而纤维直径分布未改变。
