Yoshii Satoru, Shima Mitsuhiro, Oka Masanori, Taniguchi Ataru, Taki Yoshiro, Akagi Masao
Institute of Biomedical Engineering, Kansai Denryoku Hospital, Imaichi, Asahi-ku, Osaka, Japan.
Neurol Res. 2004 Mar;26(2):145-50. doi: 10.1179/016164104225013770.
This article describes the regeneration of severed peripheral nerve axons along collagen filaments in the absence of the distal nerve stump. 22-mm long nerve guides made of collagen filaments were sutured to the proximal ends of severed rat sciatic nerves. The distal ends of the guides were sutured to the distal stumps of the nerves in a group and not sutured in the other. Nerve autografts and collagen tubes were used as controls. At 8 weeks postoperatively, the mean number and the mean diameter of myelinated axons were 5491 +/- 617 (mean +/- SD) and 2.3 +/- 1.3 microns at the distal ends of the collagen filaments nerve guides those the distal ends were sutured to the distal stumps of the nerves, while in the nerve autografts these were 4837 +/- 604 and 3.3 +/- 1.4 microns. These were 1992 +/- 770 and 2.7 +/- 1.2 microns at the distal ends of the collagen-filaments guides those the distal ends were not sutured to the distal stumps of the nerves, while in the nerve autografts these were 3041 +/- 847 and 2.3 +/- 1.1 microns. No axon was found at the distal ends of the collagen tubes. The results suggested that the contact guidance and the chemotaxis guided regenerating axons along the collagen filaments.
本文描述了在没有远端神经残端的情况下，切断的周围神经轴突沿胶原纤维丝的再生情况。将由胶原纤维丝制成的22毫米长的神经导管缝合到切断的大鼠坐骨神经的近端。一组将导管的远端缝合到神经的远端残端，另一组则不缝合。使用神经自体移植和胶原管作为对照。术后8周，在胶原纤维丝神经导管远端且远端已缝合到神经远端残端的部位，有髓轴突的平均数量和平均直径分别为5491±617（平均值±标准差）和2.3±1.3微米，而在神经自体移植中，这些数值分别为4837±604和3.3±1.4微米。在胶原纤维丝导管远端且远端未缝合到神经远端残端的部位，这些数值分别为1992±770和2.7±1.2微米，而在神经自体移植中，这些数值分别为3041±847和2.3±1.1微米。在胶原管远端未发现轴突。结果表明，接触导向和趋化作用引导再生轴突沿着胶原纤维丝生长。
