BAXTER J H, GOODMAN H C
J Exp Med. 1956 Oct 1;104(4):487-90.
A soluble protective factor, capable of absorbing nephrotoxic antibodies from anti-rat kidney serum, can be obtained in high concentration by tryptic digestion of rat kidney homogenate. The factor is no longer antigenic or at most only slightly so. It is stable at 60 degrees , destroyed by boiling, is non-dialyzable, can be precipitated by ammonium sulfate, but resists destruction by proteolytic and other enzymes. In accord with previous studies on the organ localization of the rat tissue antigen responsible for the production of nephrotoxic antibodies, the soluble protective factor, or antigen derivative, can be obtained by tryptic digestion of rat lung, skeletal muscle, heart, and liver.
一种可溶性保护因子,能够从抗大鼠肾脏血清中吸收肾毒性抗体,通过对大鼠肾脏匀浆进行胰蛋白酶消化可高浓度获得。该因子不再具有抗原性,或者至多仅有轻微抗原性。它在60摄氏度时稳定,煮沸可将其破坏,不可透析,能被硫酸铵沉淀,但能抵抗蛋白水解酶和其他酶的破坏。与先前关于负责产生肾毒性抗体的大鼠组织抗原的器官定位研究一致,通过对大鼠肺、骨骼肌、心脏和肝脏进行胰蛋白酶消化可获得可溶性保护因子或抗原衍生物。