Nishikawa M, Shirakawa S
Second Department of Internal Medicine, Mie University School of Medicine, Tsu, Japan.
Leuk Lymphoma. 1992 Oct;8(3):201-11. doi: 10.3109/10428199209054906.
Protein kinase C (PKC) activation and/or modulation of its isoenzyme expression play key roles in regulating the response of haematopoietic cells to both growth factors and non-physiological inducers of cell growth and differentiation. The level of PKC activities for both cytosol and particulate fractions of ALL and CLL cells are lower than those of AML type. Atypical AML blasts expressing T-cell associated CD2 and CD7 determinants have significantly lower PKC activities compared to typical AML blasts. Analyses of PKC isoforms (-alpha, -beta, and -gamma) show considerable variation with respect to leukaemic cell distributions and subcellular localisations. PKC-alpha and -beta are usually the major species in cytosolic fractions, whereas PKC-gamma is the predominant type in particulate fractions. All lymphoid cells express PKC-gamma in the cytosol, albeit as a minor component, while the occurrence of cytosol PKC-gamma in AML cells appears to be associated in particular with a typical lymphoid antigen expression.
蛋白激酶C（PKC）的激活及其同工酶表达的调节在调控造血细胞对生长因子以及细胞生长和分化的非生理性诱导剂的反应中起着关键作用。急性淋巴细胞白血病（ALL）和慢性淋巴细胞白血病（CLL）细胞的胞质和颗粒部分的PKC活性水平均低于急性髓细胞白血病（AML）类型。与典型AML原始细胞相比，表达T细胞相关CD2和CD7决定簇的非典型AML原始细胞的PKC活性显著降低。对PKC同工型（-α、-β和-γ）的分析显示，在白血病细胞分布和亚细胞定位方面存在相当大的差异。PKC-α和-β通常是胞质部分的主要类型，而PKC-γ是颗粒部分的主要类型。所有淋巴细胞在胞质中均表达PKC-γ，尽管含量较少，而AML细胞中胞质PKC-γ的出现似乎尤其与典型的淋巴细胞抗原表达有关。
