一种用于检测钙调蛋白、钙离子（II）和铕离子（III）对植物NAD激酶激活作用的连续分光光度法测定。

A continuous spectrophotometric assay for the activation of plant NAD kinase by calmodulin, calcium(II), and europium(III) ions.

作者信息

Amann B T, Mulqueen P, Horrocks W D

机构信息

Department of Chemistry, Pennsylvania State University, University Park 16802.

出版信息

J Biochem Biophys Methods. 1992 Dec;25(4):207-17. doi: 10.1016/0165-022x(92)90015-3.

DOI:10.1016/0165-022x(92)90015-3

PMID:1337352

Abstract

A continuous spectrophotometric assay has been developed to quantify the calmodulin, calcium(II) ion, and europium(III) ion dependence of the activation of NAD kinase from pea seedlings. Experimental enzyme activation data are compared with the theoretical curves for the binding of calcium(II) ions to the individual calcium binding sites of calmodulin. These results indicate that the binding of three calcium(II) ions is necessary for activation of plant NAD kinase. Further studies demonstrate that europium(III) ions can replace calcium(II) ions in calmodulin with retention of its ability to activate NAD kinase.

摘要

已开发出一种连续分光光度测定法，用于定量豌豆幼苗中NAD激酶激活对钙调蛋白、钙离子（II）和铕离子（III）的依赖性。将实验性酶激活数据与钙离子（II）与钙调蛋白各个钙结合位点结合的理论曲线进行比较。这些结果表明，三个钙离子（II）的结合是激活植物NAD激酶所必需的。进一步的研究表明，铕离子（III）可以取代钙调蛋白中的钙离子（II），并保留其激活NAD激酶的能力。