Beebe A M, Gluckstern T G, George J, Pedersen N C, Dandekar S
Department of Internal Medicine, School of Medicine, University of California, Davis 95616.
Vet Immunol Immunopathol. 1992 Dec;35(1-2):37-49. doi: 10.1016/0165-2427(92)90119-b.
Natural or experimental feline immunodeficiency virus (FIV) infection in cats is often associated with hematologic abnormalities which are similar to those observed in human immunodeficiency virus (HIV) infected patients. To determine if cells in bone marrow are infected with FIV and whether severity of hematopoietic disorder is correlated with the level of viral infection, bone marrow tissues from ten experimentally and two naturally FIV infected cats were examined by in situ hybridization for presence of FIV RNA. Seven of the 12 FIV infected cats were also naturally or experimentally coinfected with feline leukemia virus (FeLV). FIV RNA was detected mainly in megakaryocytes and unidentified mononuclear cells in the bone marrow of cats that were sick and had marrow hypercellularity and immaturity. These included all cats in the acute phase of FIV infection and two of seven long term FIV infected cats. One long term FIV infected cat with lymphosarcoma was also positive for FIV RNA in bone marrow cells. The other four long term FIV infected cats were relatively healthy, with normal bone marrow morphology, and were negative for FIV infected cells. Bone marrow from three non-infected and two cats infected with FeLV alone were also negative for FIV RNA by in situ hybridization. We concluded that megakaryocytes and mononuclear cells were targets of the viral infection and that the presence of FIV RNA in cells of the bone marrow correlated with marrow hypercellularity and immaturity, and severity of illness.
猫自然感染或实验性感染猫免疫缺陷病毒（FIV）通常与血液学异常有关，这些异常与人类免疫缺陷病毒（HIV）感染患者中观察到的异常相似。为了确定骨髓中的细胞是否感染了FIV，以及造血系统疾病的严重程度是否与病毒感染水平相关，对10只实验性感染和2只自然感染FIV的猫的骨髓组织进行了原位杂交，以检测FIV RNA的存在。12只感染FIV的猫中有7只也自然感染或实验性感染了猫白血病病毒（FeLV）。FIV RNA主要在患病且骨髓细胞增多和不成熟的猫的骨髓巨核细胞和未鉴定的单核细胞中检测到。这些猫包括所有处于FIV感染急性期的猫以及7只长期感染FIV的猫中的2只。一只患有淋巴瘤的长期感染FIV的猫的骨髓细胞中FIV RNA也呈阳性。其他4只长期感染FIV的猫相对健康，骨髓形态正常，FIV感染细胞呈阴性。3只未感染和2只仅感染FeLV的猫的骨髓原位杂交FIV RNA也呈阴性。我们得出结论，巨核细胞和单核细胞是病毒感染的靶细胞，骨髓细胞中FIV RNA的存在与骨髓细胞增多、不成熟及疾病严重程度相关。
