Hudson Lola C, Tompkins Mary B, Meeker Rick B
Department of Molecular Biomedical Sciences, College of Veterinary Medicine, 4700 Hillsborough Street, Raleigh, NC 27606, USA.
Cell Tissue Res. 2008 Oct;334(1):55-65. doi: 10.1007/s00441-008-0623-7. Epub 2008 Jul 30.
Trafficking of peripheral blood mononuclear cells (PBMCs) into the brain is a critical step in the initiation of human immunodeficiency virus (HIV)-associated central nervous system disease. To examine potential factors that control trafficking during the earliest stages of infection, PBMC transmigration across a cultured feline brain endothelial cell (BECs) monolayer was measured after selective exposure of various cell types to feline immunodeficiency virus (FIV). Infection of the PBMCs with FIV increased the trafficking of monocytes and CD4 and CD8 T cells. Additional exposure of the BECs to FIV suppressed mean monocyte, CD4 T cell, and CD8 T cell trafficking. B cell trafficking was unaltered by these changing conditions. Subsequent exposure of astrocytes or microglia to FIV altered transmigration of different PBMC subsets in different ways. Treated microglia compared with treated astrocytes decreased monocyte transmigration, whereas B cell transmigration was increased significantly. When both astrocytes and microglia were exposed to FIV, an increase in CD8 T cell transmigration relative to BECs alone, to BECs plus astrocytes, or to BECs plus microglia was demonstrated. Thus, initial exposure of PBMCs to FIV is sufficient to induce a general increase in trafficking, whereas initial exposure of endothelial cells to FIV tends to down-regulate this effect. Selectivity of trafficking of specific PBMC subsets is apparent only after exposure of cells of the central nervous system to FIV in co-culture with the endothelium.
外周血单个核细胞（PBMC）向脑内的迁移是人类免疫缺陷病毒（HIV）相关中枢神经系统疾病发病过程中的关键步骤。为了研究感染早期控制迁移的潜在因素，在将各种细胞类型选择性暴露于猫免疫缺陷病毒（FIV）后，检测PBMC跨培养的猫脑内皮细胞（BEC）单层的迁移情况。FIV感染PBMC增加了单核细胞以及CD4和CD8 T细胞的迁移。BEC进一步暴露于FIV会抑制单核细胞、CD4 T细胞和CD8 T细胞的平均迁移。这些变化条件未改变B细胞的迁移。随后将星形胶质细胞或小胶质细胞暴露于FIV会以不同方式改变不同PBMC亚群的迁移。与经处理的星形胶质细胞相比，经处理的小胶质细胞会减少单核细胞迁移，而B细胞迁移则显著增加。当星形胶质细胞和小胶质细胞都暴露于FIV时，相对于单独的BEC、BEC加星形胶质细胞或BEC加小胶质细胞，CD8 T细胞迁移增加。因此，PBMC最初暴露于FIV足以诱导迁移普遍增加，而内皮细胞最初暴露于FIV则倾向于下调这种效应。只有在中枢神经系统细胞与内皮细胞共培养中暴露于FIV后，特定PBMC亚群迁移的选择性才会显现出来。
