Levkutova M, Levkut M, Bajová V
Department of Infectious and Tropical Diseases, University of Veterinary Medicine, Kosice, Tschecho-Slovakia.
Folia Histochem Cytobiol. 1992;30(3):103-5.
Labelling of peripheral blood Lymphocytes surface antigens was carried out using the method of colloidal gold, enhanced with silver staining. Instead of PBS the minimal essential medium (MEM) according the Eagle, pH 7.2, was used rinsing of isolated lymphocytes. Visibility of positive reactions on lymphocytes at application of both mentioned media was the same. Positive reaction at demonstration of p24 BLV on cells acquired the from of black cap while the IgG expression was observed in the from of diffuse dispersion of colloidal gold on cells. Differences between the application of individual media were observed in the shape of peripheral lymphocytes in smears. Utilization of Eagle's MEM resulted in more uniform shapes and optically smooth surfaces when viewed under a light microscope.
采用胶体金法对外周血淋巴细胞表面抗原进行标记，并用银染色增强。分离的淋巴细胞冲洗时，使用的不是磷酸盐缓冲液（PBS），而是依 Eagle 配方的最低必需培养基（MEM），pH 值为 7.2。使用上述两种培养基时，淋巴细胞上阳性反应的可见度相同。在细胞上检测 p24 BLV 时的阳性反应呈黑帽状，而 IgG 表达则表现为细胞上胶体金的弥漫性分散。涂片中外周淋巴细胞的形态在使用不同培养基时存在差异。在光学显微镜下观察，使用 Eagle's MEM 时淋巴细胞形态更均匀，表面更光滑。
