De Clercq E, Janik B, Sommer R G
Chem Biol Interact. 1976 Jul;14(1-2):113-25. doi: 10.1016/0009-2797(76)90029-6.
The interferon-inducing activity of the double-stranded complex poly(A) - poly(U) in primary rabbit kidney cell cultures is reduced when the cells are treated with poly(dUfl) either 1 h before, simultaneously with, or 1 h after the exposure to the double-stranded complex. It has been demonstrated in experiments involving sensitivity to hydrolysis by RNAase, UV absorbance-mixing curves, and UV absorbance-temperature profiles that this phenomenon is due to the formation of the triple-stranded complex poly(A) - poly(U) - poly(dUfl). The latter complex seems to be the principal product of interactions in the following systems: poly(A) - poly(U) + poly(dUfl); poly(A) - poly(dUfl) + poly(U); and poly(A) + poly(U) + poly (dUfl).
当原代兔肾细胞培养物在暴露于双链复合物聚（A）-聚（U）之前1小时、同时或之后1小时用聚（dUfl）处理时，双链复合物聚（A）-聚（U）在这些细胞培养物中的干扰素诱导活性会降低。在涉及对核糖核酸酶水解的敏感性、紫外吸收混合曲线以及紫外吸收温度曲线的实验中已证明，这种现象是由于形成了三链复合物聚（A）-聚（U）-聚（dUfl）。后一种复合物似乎是以下体系中相互作用的主要产物：聚（A）-聚（U）+聚（dUfl）；聚（A）-聚（dUfl）+聚（U）；以及聚（A）+聚（U）+聚（dUfl）。
