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对固定和未固定组织切片中冻干酶和桥粒酶进行定量评估。

Quantitative estimation of lyo- and desmoenzymes in tissue sections with and without fixation.

作者信息

NACHLAS M M, PRINN W, SELIGMAN A M

出版信息

J Biophys Biochem Cytol. 1956 Sep 25;2(5):487-502. doi: 10.1083/jcb.2.5.487.

Abstract
  1. Tissue sections eight microns thick were exposed to various experimental conditions used in histochemistry, and the effect upon the activities of esterase, the phosphatases, leucine aminopeptidase, beta-glucuronidase, and arylsulfatase was determined colorimetrically. 2. Significant differences were found in the amounts of the lyo and desmo fractions of these enzymes. The desmo components were found to be for esterase, alkaline phosphatase, leucine aminopeptidase, acid phosphatase, beta-glucuronidase, and arylsulfatase, (1/3), 2/3, 2/3, (1/2), (1/8), and (1/8) of the total enzymatic activity respectively. 3. Variations in the time and in the temperature at which diffusion was studied and of the pH and salt concentration of the solution into which the sections were placed, resulted in differences in the amount of enzymatic activity which remained in the tissue section. Some enzyme loss by diffusion was noted even after fixation of the tissue section. 4. The significance of the findings with respect to some of the concepts of localization of enzymes in tissue sections was discussed.
摘要
  1. 将八微米厚的组织切片置于组织化学中使用的各种实验条件下,通过比色法测定其对酯酶、磷酸酶、亮氨酸氨肽酶、β-葡萄糖醛酸酶和芳基硫酸酯酶活性的影响。2. 发现这些酶的溶解部分和结合部分的量存在显著差异。结合成分分别占酯酶、碱性磷酸酶、亮氨酸氨肽酶、酸性磷酸酶、β-葡萄糖醛酸酶和芳基硫酸酯酶总酶活性的(1/3)、2/3、2/3、(1/2)、(1/8)和(1/8)。3. 研究扩散的时间和温度以及切片浸入溶液的pH值和盐浓度的变化,导致组织切片中残留的酶活性量有所不同。即使在组织切片固定后,也注意到有一些酶因扩散而损失。4. 讨论了这些发现对于组织切片中酶定位的一些概念的意义。

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Quantitative estimation of lyo- and desmoenzymes in tissue sections with and without fixation.
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本文引用的文献

1
The histochemical demonstration of esterase.
J Natl Cancer Inst. 1949 Apr-Jun;9(5-6):415-25.
3
Effect of formalin fixation on the activity of five enzymes of rat liver.
Stain Technol. 1951 Jan;26(1):19-23. doi: 10.3109/10520295109113172.
4
Histochemical demonstration of beta-d-glucuronidase with a synthetic substrate.
J Histochem Cytochem. 1954 May;2(3):209-29. doi: 10.1177/2.3.209.
5
Histochemical demonstration of aryl sulfatase.
Science. 1952 Nov 14;116(3020):539-43. doi: 10.1126/science.116.3020.539.

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