Robinson P M, Bell C
J Cell Biol. 1967 Apr;33(1):93-102. doi: 10.1083/jcb.33.1.93.
Acetylcholinesterase has been localized at the autonomic neuromuscular junction in the bladder of the toad (Bufo marinus) by the Karnovsky method. High levels of enzyme activity have been demonstrated in association with the membranes of cholinergic axons and the adjacent membranes of the accompanying Schwann cells. The synaptic vesicles stained in occasional cholinergic axons. After longer incubation times, the membrane of smooth muscle cells close to cholinergic axons also stained. Axons with only moderate acetylcholinesterase activity or with no activity at all were seen in the same bundles as cholinergic axons, but identification of the transmitter in these axons was not possible.
采用卡诺夫斯基方法已将乙酰胆碱酯酶定位在蟾蜍（海蟾蜍）膀胱的自主神经肌肉接头处。已证实在胆碱能轴突膜以及伴随的施万细胞的相邻膜上存在高水平的酶活性。偶尔有胆碱能轴突中的突触小泡被染色。孵育时间延长后，靠近胆碱能轴突的平滑肌细胞膜也被染色。在与胆碱能轴突相同的神经束中可见到乙酰胆碱酯酶活性仅为中等或完全没有活性的轴突，但无法确定这些轴突中的递质。
