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Enzymatic synthesis of PAPS with an ATP-regeneration system.

作者信息

Ibuki H, Tashiro T, Hayashi M, Nakajima H, Liu M C, Suiko M

机构信息

Biochemistry Department, Unitika Research and Development Center, Kyoto, Japan.

出版信息

Nucleic Acids Symp Ser. 1992(27):171-2.

PMID:1337784
Abstract

Sulfate activating enzymes, ATP sulfurylase and APS kinase, were newly isolated from a thermophile, Bacillus stearothermophilus. Adenosine 3'-phosphate 5'-phosphosulfate (PAPS) was synthesized by these enzymes. The reaction proceeded more efficiently when an ATP-regeneration system, using acetate kinase, was coupled to the reaction system.

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