Ibuki H, Tashiro T, Hayashi M, Nakajima H, Liu M C, Suiko M
Biochemistry Department, Unitika Research and Development Center, Kyoto, Japan.
Nucleic Acids Symp Ser. 1992(27):171-2.
Sulfate activating enzymes, ATP sulfurylase and APS kinase, were newly isolated from a thermophile, Bacillus stearothermophilus. Adenosine 3'-phosphate 5'-phosphosulfate (PAPS) was synthesized by these enzymes. The reaction proceeded more efficiently when an ATP-regeneration system, using acetate kinase, was coupled to the reaction system.