• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Recognition of model DNA replication forks by the SV40 large tumor antigen.

作者信息

SenGupta D J, Blackwell L J, Gillette T, Borowiec J A

机构信息

Department of Biochemistry, New York University Medical Center, New York 10016.

出版信息

Chromosoma. 1992;102(1 Suppl):S46-51. doi: 10.1007/BF02451785.

DOI:10.1007/BF02451785
PMID:1337879
Abstract

The ability of the SV40 large tumor antigen (T antigen), a DNA helicase, to bind to model DNA replication forks was tested. DNA fork molecules were constructed either from two partially complementary oligonucleotides or from a single oligonucleotide able to form a 'panhandle' structure. T antigen specifically recognized the two-strand fork in a reaction dependent on the presence of ATP, dATP, or non-hydrolyzable analogs of ATP. T antigen asymmetrically bound the two-strand fork, protecting from nuclease cleavage a fork-proximal region on only one of the two strands. The asymmetric binding is consistent with the 3'-->5' directionality of the DNA helicase activity of T antigen. An analogous region on the one-strand fork was also bound by T antigen, suggesting that T antigen does not require a free single-stranded end to load onto the fork. Use of chemically modified DNA substrates indicated that T antigen binding to the fork utilized important contacts with the DNA sugar-phosphate backbone.

摘要

相似文献

1
Recognition of model DNA replication forks by the SV40 large tumor antigen.
Chromosoma. 1992;102(1 Suppl):S46-51. doi: 10.1007/BF02451785.
2
Strand-specific recognition of a synthetic DNA replication fork by the SV40 large tumor antigen.
Science. 1992 Jun 19;256(5064):1656-61. doi: 10.1126/science.256.5064.1656.
3
Dimerization of simian virus 40 T-antigen hexamers activates T-antigen DNA helicase activity.猿猴病毒40 T抗原六聚体的二聚化激活了T抗原DNA解旋酶活性。
J Virol. 1997 Nov;71(11):8766-73. doi: 10.1128/JVI.71.11.8766-8773.1997.
4
Characterization of simian virus 40 T-antigen double hexamers bound to a replication fork. The active form of the helicase.与复制叉结合的猿猴病毒40 T抗原双六聚体的表征。解旋酶的活性形式。
J Biol Chem. 2002 Nov 22;277(47):44886-97. doi: 10.1074/jbc.M207022200. Epub 2002 Sep 19.
5
Two major replicating simian virus 40 chromosome classes. Synchronous replication fork movement is associated with bound large T antigen during elongation.两种主要的复制型猿猴病毒40染色体类别。在延伸过程中，同步复制叉移动与结合的大T抗原相关联。
J Biol Chem. 1987 May 5;262(13):6339-49.
6
UV lesions located on the leading strand inhibit DNA replication but do not inhibit SV40 T-antigen helicase activity.位于前导链上的紫外线损伤会抑制DNA复制，但不会抑制SV40 T抗原解旋酶活性。
Mutat Res. 2000 Feb 16;459(1):19-28. doi: 10.1016/s0921-8777(99)00052-x.
7
Escherichia coli PriA helicase: fork binding orients the helicase to unwind the lagging strand side of arrested replication forks.大肠杆菌PriA解旋酶：叉形结构结合使解旋酶定向，以解开停滞复制叉的滞后链一侧。
J Mol Biol. 2001 Oct 5;312(5):935-47. doi: 10.1006/jmbi.2001.4930.
8
Synthetic DNA replication bubbles bound and unwound with twofold symmetry by a simian virus 40 T-antigen double hexamer.合成的DNA复制泡被猿猴病毒40 T抗原双六聚体以双重对称性结合并解旋。
J Virol. 1998 Nov;72(11):8676-81. doi: 10.1128/JVI.72.11.8676-8681.1998.
9
Murine p53 inhibits the function but not the formation of SV40 T antigen hexamers and stimulates T antigen RNA helicase activity.小鼠p53抑制SV40 T抗原六聚体的功能而非其形成，并刺激T抗原RNA解旋酶活性。
Chromosoma. 1992;102(1 Suppl):S60-6. doi: 10.1007/BF02451787.
10
Strand specificity in the interactions of Escherichia coli primary replicative helicase DnaB protein with a replication fork.大肠杆菌主要复制解旋酶DnaB蛋白与复制叉相互作用中的链特异性
Biochemistry. 1997 Aug 19;36(33):10320-6. doi: 10.1021/bi970712a.

引用本文的文献

1
Single 8-oxo-guanine and 8-oxo-adenine lesions induce marked changes in the backbone structure of a 25-base DNA strand.单个8-氧代鸟嘌呤和8-氧代腺嘌呤损伤会导致一条25个碱基的DNA链的主链结构发生显著变化。
Proc Natl Acad Sci U S A. 2000 Nov 7;97(23):12442-5. doi: 10.1073/pnas.230438797.
2
The origin DNA-binding and single-stranded DNA-binding domains of simian virus 40 large T antigen are distinct.猿猴病毒40大T抗原的起始DNA结合域和单链DNA结合域是不同的。
J Virol. 1998 Dec;72(12):10256-9. doi: 10.1128/JVI.72.12.10256-10259.1998.
3
Strand and face: the topography of interactions between the SV40 origin of replication and T-antigen during the initiation of replication.

本文引用的文献

1
Studies on DNA replication in the bacteriophage T4 in vitro system.噬菌体T4体外系统中DNA复制的研究。
Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:655-68. doi: 10.1101/sqb.1983.047.01.077.
2
Initiation of simian virus 40 DNA replication in vitro: large-tumor-antigen- and origin-dependent unwinding of the template.体外猿猴病毒40 DNA复制的起始：模板的大肿瘤抗原及起源依赖性解旋
Proc Natl Acad Sci U S A. 1987 Jun;84(11):3643-7. doi: 10.1073/pnas.84.11.3643.
3
Replication of simian virus 40 origin-containing DNA in vitro with purified proteins.
链与面：复制起始过程中SV40复制起点与T抗原之间相互作用的拓扑结构。
EMBO J. 1994 Feb 15;13(4):982-92. doi: 10.1002/j.1460-2075.1994.tb06343.x.
用纯化蛋白在体外复制含猿猴病毒40起始位点的DNA。
Proc Natl Acad Sci U S A. 1987 Apr;84(7):1834-8. doi: 10.1073/pnas.84.7.1834.
4
Simian virus 40 (SV40) DNA replication: SV40 large T antigen unwinds DNA containing the SV40 origin of replication.猿猴病毒40（SV40）的DNA复制：SV40大T抗原解开含有SV40复制起点的DNA。
Proc Natl Acad Sci U S A. 1987 Jan;84(1):16-20. doi: 10.1073/pnas.84.1.16.
5
DNA helicase activity of SV40 large tumor antigen.SV40大肿瘤抗原的DNA解旋酶活性
EMBO J. 1986 Aug;5(8):1939-44. doi: 10.1002/j.1460-2075.1986.tb04447.x.
6
Role of DNA polymerase alpha and DNA primase in simian virus 40 DNA replication in vitro.DNA聚合酶α和DNA引发酶在猴病毒40体外DNA复制中的作用。
Proc Natl Acad Sci U S A. 1986 May;83(9):2869-73. doi: 10.1073/pnas.83.9.2869.
7
Localized melting and structural changes in the SV40 origin of replication induced by T-antigen.
EMBO J. 1988 Oct;7(10):3149-58. doi: 10.1002/j.1460-2075.1988.tb03182.x.
8
Cellular factors required for multiple stages of SV40 DNA replication in vitro.体外SV40 DNA复制多个阶段所需的细胞因子。
EMBO J. 1988 Apr;7(4):1211-8. doi: 10.1002/j.1460-2075.1988.tb02933.x.
9
Purification and characterization of replication protein A, a cellular protein required for in vitro replication of simian virus 40 DNA.复制蛋白A的纯化与特性分析，一种猿猴病毒40 DNA体外复制所需的细胞蛋白。
Proc Natl Acad Sci U S A. 1988 Apr;85(8):2523-7. doi: 10.1073/pnas.85.8.2523.
10
ATP-dependent formation of a specialized nucleoprotein structure by simian virus 40 (SV40) large tumor antigen at the SV40 replication origin.猿猴病毒40（SV40）大T抗原在SV40复制起点通过ATP依赖性方式形成一种特殊的核蛋白结构。
Proc Natl Acad Sci U S A. 1987 Dec;84(24):8981-5. doi: 10.1073/pnas.84.24.8981.