Balcarová Z, Mrázek J, Kleinwächter V, Brabec V
Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.
Gen Physiol Biophys. 1992 Dec;11(6):579-88.
The effect of binding of an antitumour drug cis-diamminedichloroplatinum(II) (cis-[Pt(NH3)2Cl2]) to DNA on cutting effectiveness of BamHI, EcoRI, and SalI restriction endonucleases was quantitatively determined. The platinum complex inhibits the cleavage of plasmid pHC624 DNA linearized by BglI restrictase. From the present results we conclude that the yield of restriction endonuclease cleavage is also lowered if the platinum complex is bound outside the recognition DNA sequence of these enzymes. We propose that the origin of platinum adducts on DNA outside the recognition sequence can decrease the yield of restriction enzyme cleavage via inducing a conformational perturbation in the recognition DNA sequence of these enzymes and also via inhibition of the linear diffusion of these enzymes on DNA.
定量测定了抗肿瘤药物顺二氯二氨合铂（II）（顺式-[Pt(NH₃)₂Cl₂]）与DNA结合对BamHI、EcoRI和SalI限制性内切酶切割效率的影响。铂配合物抑制了由BglI限制酶线性化的质粒pHC624 DNA的切割。从目前的结果我们得出结论，如果铂配合物结合在这些酶的识别DNA序列之外，限制性内切酶切割的产量也会降低。我们提出，识别序列之外DNA上铂加合物的产生可通过诱导这些酶识别DNA序列的构象扰动以及抑制这些酶在DNA上的线性扩散来降低限制酶切割的产量。
