人乳头瘤病毒16型基因在子宫颈癌中的发生与表达

Occurrence and expression of human papillomavirus type 16 genes in uterine cervical carcinomas.

作者信息

Tanimoto H

机构信息

Department of Obstetrics and Gynecology, Hiroshima University School of Medicine, Japan.

出版信息

Hiroshima J Med Sci. 1992 Dec;41(4):71-7.

PMID:1338065

Abstract

The detection of human papillomavirus (HPV) type 16 early genes: E7, E5, and the late gene: L1 was attempted in 42 uterine cervical neoplasia (35 cervical carcinomas and 7 cervical dysplasias) using the polymerase chain reaction (PCR) method. Consequently, E7 gene was detected in 19 (54.3%) of 35 carcinomas and in 5 (71.4%) of 7 dysplasias, E5 gene was detected in 7 (20.0%) of 35 carcinomas and in 5 (71.4%) of 7 dysplasias, L1 gene was detected in 18 (51.4%) of 35 carcinomas and in 5 (71.4%) of 7 dysplasias, respectively. In order to elucidate the transcriptional pattern of HPV type 16 in each of the clinical stages, the expression of mRNA for E7, E5 and L1 genes was examined in HPV DNA positive cases using the reverse transcriptase polymerase chain reaction (RT-PCR) method. E7 gene mRNA was detected in 18 (94.7%) of 19 cervical carcinomas, whereas E5 and L1 genes mRNAs were detected in only 4 (57.1%) of 7 and in one (5.6%) of 18 carcinomas respectively. In cervical dysplasias, E7, E5 and L1 genes mRNA were detected in all cases. E7, E5 and L1 genes were transcriptionally active in all dysplasias, whereas E5 and L1 genes were not always transcriptionally active in carcinomas. These results suggest that the HPV type 16 early gene E7 is present preferentially as integrated form and transcriptionally active in the carcinoma cell, and plays an important role in the development of malignancy. On the other hand, E5 and L1 genes are present and transcribed in the dysplasia cell but their transcriptional activity is less frequent in the carcinoma cell.

摘要

采用聚合酶链反应（PCR）方法，对42例子宫颈肿瘤（35例宫颈癌和7例宫颈发育异常）进行了人乳头瘤病毒16型（HPV-16）早期基因E7、E5及晚期基因L1的检测。结果显示，在35例宫颈癌中有19例（54.3%）检测到E7基因，7例宫颈发育异常中有5例（71.4%）检测到E7基因；35例宫颈癌中有7例（20.0%）检测到E5基因，7例宫颈发育异常中有5例（71.4%）检测到E5基因；35例宫颈癌中有18例（51.4%）检测到L1基因，7例宫颈发育异常中有5例（71.4%）检测到L1基因。为阐明HPV-16在各临床阶段的转录模式，采用逆转录聚合酶链反应（RT-PCR）方法，对HPV DNA阳性病例的E7、E5和L1基因的mRNA表达进行了检测。在19例宫颈癌中，有18例（94.7%）检测到E7基因mRNA，而在7例中有4例（57.1%）检测到E5基因mRNA，在18例中有1例（5.6%）检测到L1基因mRNA。在宫颈发育异常病例中，所有病例均检测到E7、E5和L1基因mRNA。E7、E5和L1基因在所有发育异常病例中均具有转录活性，而E5和L1基因在癌组织中并非总是具有转录活性。这些结果表明，HPV-16早期基因E7在癌细胞中优先以整合形式存在且具有转录活性，在恶性肿瘤的发生发展中起重要作用。另一方面，E5和L1基因在发育异常细胞中存在并转录，但在癌细胞中的转录活性较低。