Kofoed J A, Barcelo A C, Tocci A A
Experientia. 1976;32(7):851-2. doi: 10.1007/BF02003724.
Urine specimens from healthy children were concentrated and filtered through Sephadex G-100 and G-25. The eluate was recovered in fractions of 5 ml each. Total hexoses, galactose, total proteins and total uronic acids were determined on each fraction. Total hexoses and galactose were eluted in 6 distinct peaks. The uronic acid containing GAG were eluted in 4 small peaks on G-25 and 1 peak on G-100. This peak is eluted always with high amounts of galactose containing glycoproteins.
收集健康儿童的尿液样本，通过葡聚糖G - 100和G - 25进行浓缩和过滤。洗脱液以每份5毫升的馏分进行收集。对每个馏分测定总己糖、半乳糖、总蛋白和总糖醛酸。总己糖和半乳糖在6个不同的峰中洗脱。含糖醛酸的糖胺聚糖在G - 25上以4个小峰洗脱，在G - 100上以1个峰洗脱。这个峰总是与大量含半乳糖的糖蛋白一起洗脱。
