Wigle D A, Bennett B M, Jennings D B, Sarda I R, Flynn T G, Pang S C
Department of Anatomy, Queen's University, Kingston, Ont., Canada.
Can J Physiol Pharmacol. 1992 Nov;70(11):1525-8. doi: 10.1139/y92-218.
Rat brain natriuretic peptide (rBNP) and iso-atrial natriuretic peptide (iso-rANP) were discovered independently by two research laboratories. They are considered to be members of the B-type natriuretic peptides. Except for the Gln/Leu substitution at position 44, the amino acid sequence of iso-rANP is identical with that of the C-terminal 45 amino acids of rat pro-BNP and with the 5-kDa cardiac peptide from rat atria. To determine whether this amino acid substitution can modify the known biological effects of rBNP and iso-rANP, the present investigation examined the cardiovascular and renal responses, vasorelaxant effect, receptor binding characteristics, and cyclic GMP production by the two peptides in relation to that of rat atrial natriuretic peptide (rANP). Results indicate that rBNP and iso-rANP are indistinguishable from each other in terms of these known biological activities of atrial natriuretic peptide. We therefore conclude that rBNP and iso-rANP are identical peptides and that the amino acid substitution at position 44 represents a polymorphic form of the rat B-type natriuretic peptide.
大鼠脑钠肽（rBNP）和异心房钠尿肽（iso-rANP）是由两个研究实验室分别独立发现的。它们被认为是B型钠尿肽家族的成员。除了第44位的谷氨酰胺/亮氨酸替换外，iso-rANP的氨基酸序列与大鼠pro-BNP的C末端45个氨基酸以及大鼠心房的5 kDa心脏肽的氨基酸序列相同。为了确定这种氨基酸替换是否会改变rBNP和iso-rANP已知的生物学效应，本研究考察了这两种肽与大鼠心房钠尿肽（rANP）相关的心血管和肾脏反应、血管舒张作用、受体结合特性以及环磷酸鸟苷的产生。结果表明，就心房钠尿肽的这些已知生物学活性而言，rBNP和iso-rANP彼此难以区分。因此，我们得出结论，rBNP和iso-rANP是相同的肽，并且第44位的氨基酸替换代表了大鼠B型钠尿肽的一种多态形式。
