Yaguchi Y, Tomino Y, Ozaki T, Okumura K, Sendo F, Koide H
Department of Medicine, Juntendo University School of Medicine, Tokyo, Japan.
Nephron. 1992;62(4):444-8. doi: 10.1159/000187096.
The effects of the reduction of neutrophils in glomeruli on the improvement of proteinuria and glomerular injuries were determined in the first (heterologous) phase of Masugi (nephrotoxic) nephritis. Male (6-week-old) WKA/Hkm rats were initially injected with 2.0 ml of a newly developed monoclonal antineutrophil antibody and then injected with 1.0 ml of nephrotoxins. This monoclonal anti-neutrophil antibody was found to have selectively reduced the number of neutrophils in the glomerular capillary lumen in cases of Masugi nephritis by light microscopy. Malondialdehyde (MDA) levels or superoxide dismutase (SOD) activities in renal tissues of such rats were also examined. However, there were no significant differences in the levels of proteinuria and the number of glomerular cells containing resident cells and infiltrated mononuclear cells in the first phase of Masugi nephritis with or without pretreatment with antineutrophil antibody. No significant differences were observed in the levels of MDA or SOD activities in renal tissues of Masugi nephritis with or without pretreatment with such an antibody either. It appeared that infiltration of neutrophils in the glomeruli might not be related to proteinuria and glomerular injuries in the first phase of Masugi nephritis. It was postulated that the massive proteinuria in the first phase of Masugi nephritis might be correlated with the activities of reactive oxygen species induced by the glomerular cells, i.e. glomerular resident cells and infiltrated mononuclear cells.
在Masugi（肾毒性）肾炎的第一（异源）阶段，确定了肾小球中嗜中性粒细胞减少对蛋白尿改善和肾小球损伤的影响。雄性（6周龄）WKA/Hkm大鼠最初注射2.0 ml新开发的单克隆抗中性粒细胞抗体，然后注射1.0 ml肾毒素。通过光学显微镜发现，这种单克隆抗中性粒细胞抗体在Masugi肾炎病例中选择性地减少了肾小球毛细血管腔内的中性粒细胞数量。还检测了这些大鼠肾组织中的丙二醛（MDA）水平或超氧化物歧化酶（SOD）活性。然而，在Masugi肾炎的第一阶段，无论是否用抗中性粒细胞抗体进行预处理，蛋白尿水平以及含有驻留细胞和浸润单核细胞的肾小球细胞数量均无显著差异。在Masugi肾炎的肾组织中，无论是否用这种抗体进行预处理，MDA水平或SOD活性也未观察到显著差异。看来，肾小球中嗜中性粒细胞的浸润可能与Masugi肾炎第一阶段的蛋白尿和肾小球损伤无关。据推测，Masugi肾炎第一阶段的大量蛋白尿可能与肾小球细胞（即肾小球驻留细胞和浸润单核细胞）诱导的活性氧物质的活性相关。
