编码人谷氨酸脱羧酶大型同工型的cDNA的核苷酸序列及染色体定位

Nucleotide sequence and chromosomal assignment of a cDNA encoding the large isoform of human glutamate decarboxylase.

作者信息

Kelly C D, Edwards Y, Johnstone A P, Harfst E, Nógrádi A, Nussey S S, Povey S, Carter N D

机构信息

Department of Child Health, St George's Hospital Medical School, London.

出版信息

Ann Hum Genet. 1992 Jul;56(3):255-65. doi: 10.1111/j.1469-1809.1992.tb01150.x.

DOI:10.1111/j.1469-1809.1992.tb01150.x

PMID:1339255

Abstract

Glutamic acid decarboxylase (GAD) catalyses the conversion of L-glutamic acid to the inhibitory neurotransmitter gamma-aminobutyric acid (GABA). Two forms of human GAD, GAD65 and GAD67, are encoded by two separate genes. A full length human GAD67 cDNA has been isolated from a human frontal cortex cDNA library and the nucleotide sequence determined. The GAD67 gene has been mapped to chromosome 2 using the polymerase chain reaction to amplify specifically the human sequence in rodent/human somatic cell hybrid DNA. This confirms that human GAD67 is not syntenic with the smaller GAD isoform GAD65 which has been assigned to chromosome 10. Production of polyclonal antiserum to a baculovirus-expressed GAD67 enabled immunocytological detection of GAD in the rat brain.

摘要

谷氨酸脱羧酶（GAD）催化L-谷氨酸转化为抑制性神经递质γ-氨基丁酸（GABA）。人GAD有两种形式，GAD65和GAD67，由两个不同的基因编码。已从人额叶皮质cDNA文库中分离出全长人GAD67 cDNA，并测定了核苷酸序列。利用聚合酶链反应在啮齿动物/人类体细胞杂种DNA中特异性扩增人类序列，将GAD67基因定位到2号染色体上。这证实了人GAD67与已定位到10号染色体上的较小的GAD同工型GAD65不同线。针对杆状病毒表达的GAD67产生的多克隆抗血清能够对大鼠脑中的GAD进行免疫细胞化学检测。