Hirai T, Yamashita M, Yoshikuni M, Tokumoto T, Kajiura H, Sakai N, Nagahama Y
Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki, Japan.
Dev Biol. 1992 Jul;152(1):113-20. doi: 10.1016/0012-1606(92)90161-9.
This paper reports the nucleotide and predicted amino acid sequences of the goldfish cdk2, a cognate variant of the cell cycle regulator cdc2. The predicted protein sequence shows strong homology to the other known cdk2 (88% for Xenopus and 90% for human). A monoclonal antibody against the C-terminal sequence of goldfish cdk2 recognized a 34-kDa protein in extracts from various goldfish tissues. The protein level was high in such tissues as testis and ovary containing actively dividing cells. Protein cdk2 binds to p13sucl, the fission yeast suc1+ gene product, but not to cyclin B, with which cdc2 forms a complex. The kinase activity of cdk2 increased 30-fold when oocytes matured, although its protein level did not remarkably change. Anti-cdk2 immunoprecipitates from 32P-labeled mature oocyte extracts contained a 47-kDa protein, which was not recognized by either anti-cyclin A or anti-cyclin B antibody, indicating complex formation of cdk2 with a protein other than cyclins A or B.
本文报道了金鱼cdk2的核苷酸序列和预测的氨基酸序列，cdk2是细胞周期调节因子cdc2的同源变体。预测的蛋白质序列与其他已知的cdk2具有高度同源性（爪蟾为88%，人类为90%）。一种针对金鱼cdk2 C末端序列的单克隆抗体在来自各种金鱼组织的提取物中识别出一种34 kDa的蛋白质。在含有活跃分裂细胞的组织如睾丸和卵巢中，该蛋白质水平较高。蛋白质cdk2与裂殖酵母suc1+基因产物p13sucl结合，但不与cdc2与之形成复合物的细胞周期蛋白B结合。当卵母细胞成熟时，cdk2的激酶活性增加了30倍，尽管其蛋白质水平没有明显变化。从32P标记的成熟卵母细胞提取物中进行的抗cdk2免疫沉淀含有一种47 kDa的蛋白质，该蛋白质未被抗细胞周期蛋白A或抗细胞周期蛋白B抗体识别，表明cdk2与细胞周期蛋白A或B以外的一种蛋白质形成了复合物。
