Mallinder P R, Pritchard A, Moir A
Department of Molecular Biology and Biotechnology, Krebs Institute, University of Sheffield, U.K.
Gene. 1992 Jan 2;110(1):9-16. doi: 10.1016/0378-1119(92)90438-u.
A 4.1-kb EcoRI fragment which includes the gene (gldA) encoding a glycerol dehydrogenase (G1DH; EC 1.1.1.6; glycerol:NAD oxidoreductase) from Bacillus stearothermophilus var. non-diastaticus has been cloned by virtue of its ability to restore glycerol utilisation to Escherichia coli glycerol kinase (glpK) and glycerol-3-phosphate dehydrogenase (glpD) mutants. Sequencing suggests that the gldA gene is likely to be monocistronic and encodes a protein of 39450 Da. The deduced amino acid composition and sequence of G1DH reveals that the protein is extremely similar to a characterized metal-dependent NAD-dependent G1DH from B. stearothermophilus RS93. The enzyme has limited homology to the iron-activated alcohol dehydrogenase of Zymomonas mobilis and the butanol dehydrogenase of Clostridium acetobutylicum.
一个4.1千碱基的EcoRI片段已被克隆，该片段包含来自嗜热脂肪芽孢杆菌非淀粉酶变种的编码甘油脱氢酶（G1DH；EC 1.1.1.6；甘油：NAD氧化还原酶）的基因（gldA），这是因为它能够使大肠杆菌甘油激酶（glpK）和甘油-3-磷酸脱氢酶（glpD）突变体恢复利用甘油的能力。测序表明，gldA基因可能是单顺反子的，编码一种39450道尔顿的蛋白质。推导的G1DH氨基酸组成和序列表明，该蛋白质与来自嗜热脂肪芽孢杆菌RS93的一种已鉴定的金属依赖性NAD依赖性G1DH极其相似。该酶与运动发酵单胞菌的铁激活乙醇脱氢酶和丙酮丁醇梭菌的丁醇脱氢酶具有有限的同源性。
