da Costa C A, Afonso L C, Toledo V P, Guimaraes T M, Nascimento E, Tavares C A, Mayrink W
Departamento de Analises Clinicas e Toxicologicas, Faculdade de Farmacia, UFMG, Belo Horizonte, MG, Brazil.
Parassitologia. 1992 Dec;34(1-3):45-51.
An industrialized vaccine against American cutaneous leishmaniasis was compared to a laboratory made vaccine in its ability to induce cellular and humoral immune responses in mice. No differences were observed between seric IgG levels or lymphoblastic proliferation response of mice immunized with either vaccine. Antigenic composition, evaluated by SDS-PAGE, was identical in both preparations. Protection induced in mice against a challenge with infective parasites was also compared. The level of protection obtained with the industrialized vaccine was comparable to that induced by the laboratory made preparation. The results showed that the industrialization process did not alter the efficacy of the vaccine.
将一种针对美洲皮肤利什曼病的工业化疫苗与实验室自制疫苗在诱导小鼠细胞免疫和体液免疫反应的能力方面进行了比较。在用两种疫苗免疫的小鼠的血清IgG水平或淋巴细胞增殖反应方面未观察到差异。通过SDS-PAGE评估的抗原组成在两种制剂中是相同的。还比较了在小鼠中诱导的针对感染性寄生虫攻击的保护作用。工业化疫苗获得的保护水平与实验室自制制剂诱导的保护水平相当。结果表明，工业化过程并未改变疫苗的效力。
