Magalhães V, Magalhães M, Lima R A, Tateno S, Magalhães E
Departamento de Medicina Tropical, CCS/UFPE. Recife, PE, Brasil.
Rev Inst Med Trop Sao Paulo. 1992 Mar-Apr;34(2):131-5.
Of 3250 diarrheal stools received for microbiologic diagnosis at a private clinical laboratory in Recife, Brazil, strains of Vibrio were isolated from 55 (1.7%). The study was carried out from May 1989 through May 1991. For recovering Vibrio, fecal samples were enriched in alkaline peptone water supplemented with 2% NaCl and subcultured on thiosulfate-citrate-bile salts-sucrose agar (TCBS). Of the recovered species, V. parahaemolyticus was most commonly found (24 strains), followed by V. furnissii (15 strains), V. cholerae non-01 (6 strains), V. alginolyticus (4 strains), V. fluvialis (2 strains), and Vibrio sp. (1 strain). The low isolation rate of Vibrio raises doubts about the cost-effectiveness of the use of TCBS in the routine enterobacteriologic workup of clinical laboratories.
在巴西累西腓的一家私立临床实验室接收的用于微生物学诊断的3250份腹泻粪便样本中,分离出弧菌菌株的有55份(1.7%)。该研究于1989年5月至1991年5月进行。为了分离弧菌,粪便样本在添加2%氯化钠的碱性蛋白胨水中增菌,并在硫代硫酸盐-柠檬酸盐-胆盐-蔗糖琼脂(TCBS)上进行传代培养。在分离出的菌种中,副溶血性弧菌最为常见(24株),其次是弗氏弧菌(15株)、非01群霍乱弧菌(6株)、溶藻弧菌(4株)、河流弧菌(2株)和弧菌属(1株)。弧菌的低分离率引发了对临床实验室常规肠道细菌学检查中使用TCBS的成本效益的质疑。