Pestka S
Department of Molecular Genetics and Microbiology, UMDNJ-Robert Wood Johnson Medical School, Piscataway 08854-5635.
Ann N Y Acad Sci. 1992 Oct 28;660:251-62. doi: 10.1111/j.1749-6632.1992.tb21077.x.
Antisense RNA was first an in vitro curiosity that was found to shut off protein synthesis in cell-free extracts. It was later shown to function in prokaryotic cells as a natural modulator of the synthesis of some proteins. Artificial antisense constructs can inhibit protein synthesis in prokaryotic and eukaryotic cells. To inhibit synthesis of proteins effectively, high ratios of antisense to sense RNAs are required. Thus, the challenge is to develop strategies to locate suitable targets and provide for amplification of the antisense RNA. This report provides a summary of our original work on antisense RNA.
反义RNA最初是在体外发现的一种新奇现象，它能在无细胞提取物中阻断蛋白质合成。后来发现它在原核细胞中作为某些蛋白质合成的天然调节因子发挥作用。人工构建的反义RNA可抑制原核细胞和真核细胞中的蛋白质合成。为了有效抑制蛋白质合成，需要反义RNA与正义RNA的高比例。因此，挑战在于开发定位合适靶点并实现反义RNA扩增的策略。本报告总结了我们在反义RNA方面的原创工作。
