Rakowicz-Szulczynska E, Mozdzanowski J, Lundberg T, Kaczmarski W, Speicher D
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104.
Growth Factors. 1992;6(4):337-46. doi: 10.3109/08977199209021545.
Gamma-interferon (gamma IFN) was found to induce expression of the 150,000 M(r) cell surface and the 35,000 M(r) chromatin receptors for nerve growth factor (NGF) in the SW1116 colorectal carcinoma cell line that does not express NGF receptors. In the SW707 colorectal carcinoma cell line that expresses a low level of NGF receptors, gamma IFN stimulated expression of the cell surface and the nuclear receptors. Induction of NGF receptors in SW1116 cells resulted in internalization and nuclear translocation of 125I-NGF. When NGF bound to the chromatin, ribosomal RNA synthesis was inhibited. Two-dimensional gel electrophoresis of [35S]methionine-labeled chromatin proteins indicated significant changes in chromatin protein composition in cells treated and not-treated with gamma IFN. gamma IFN effectively stimulated the expression of NGF receptors in two colorectal carcinoma cell lines, but inhibited the expression in melanoma and breast carcinoma cells. It is suggested that gamma IFN, by modulating the expression of NGF receptors may affect the NGF-dependent growth of some tumor cell lines.
在不表达神经生长因子（NGF）受体的SW1116结肠癌细胞系中，发现γ干扰素（γ-IFN）可诱导分子量为150,000的细胞表面受体以及分子量为35,000的染色质神经生长因子（NGF）受体的表达。在表达低水平NGF受体的SW707结肠癌细胞系中，γ-IFN刺激了细胞表面受体和核受体的表达。SW1116细胞中NGF受体的诱导导致125I-NGF的内化和核转位。当NGF与染色质结合时，核糖体RNA合成受到抑制。用[35S]甲硫氨酸标记的染色质蛋白的二维凝胶电泳表明，经γ-IFN处理和未处理的细胞中染色质蛋白组成有显著变化。γ-IFN有效刺激了两种结肠癌细胞系中NGF受体的表达，但抑制了黑色素瘤和乳腺癌细胞中的表达。提示γ-IFN通过调节NGF受体的表达可能影响某些肿瘤细胞系的NGF依赖性生长。
