一种针对溶组织内阿米巴的侵袭特异性单克隆抗体的进一步诊断应用。

Further diagnostic use of an invasive-specific monoclonal antibody against Entamoeba histolytica.

作者信息

González-Ruíz A, Haque R, Rehman T, Aguirre A, Castañón G, Hall A, Guhl F, Ruíz-Palacios G, Warhurst D C, Miles M A

机构信息

Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, UK.

出版信息

Arch Med Res. 1992;23(2):281-3.

PMID:1340315

Abstract

Genotypic differences between invasive and non-invasive E. histolytica could explain the 1:10 ratio of symptomatic/asymptomatic infection worldwide. Currently, zymodeme analysis is used to differentiate invasive from non-invasive E. histolytica strains but the technique is cumbersome and expensive. In accordance with the WHO research priorities for amebiasis we report here the further use of an invasive-specific monoclonal antibody against E. histolytica in immunofluorescence, to identify isolates cultured from stool samples of patients from three geographically distant endemic regions: Bangladesh, Colombia and Mexico. We tested 107 E. histolytica isolates and the correlation between zymodeme characterization and the immunofluorescence assay was 100%.

摘要

侵袭性和非侵袭性溶组织内阿米巴之间的基因型差异可以解释全球有症状/无症状感染1:10的比例。目前，酶谱分析用于区分侵袭性和非侵袭性溶组织内阿米巴菌株，但该技术繁琐且昂贵。根据世界卫生组织对阿米巴病的研究重点，我们在此报告进一步使用一种针对溶组织内阿米巴的侵袭特异性单克隆抗体进行免疫荧光检测，以鉴定从三个地理上相距遥远的流行地区（孟加拉国、哥伦比亚和墨西哥）患者粪便样本中培养的分离株。我们检测了107株溶组织内阿米巴分离株，酶谱特征与免疫荧光检测之间的相关性为100%。