Gonzalez-Ruiz A, Haque R, Rehman T, Aguirre A, Hall A, Guhl F, Warhurst D C, Miles M A
Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, United Kingdom.
J Clin Microbiol. 1994 Apr;32(4):964-70. doi: 10.1128/jcm.32.4.964-970.1994.
An invasive strain-specific monoclonal antibody against Entamoeba histolytica has been used in a capture enzyme-linked immunosorbent assay (ELISA) for the detection of invasive E. histolytica fecal antigen in clinical specimens and for the diagnosis of amebic dysentery in patients from Bangladesh. The fecal antigen capture ELISA (FAC-ELISA) did not cross-react with other parasite species in the clinical specimens or with noninvasive E. histolytica present in those specimens and in experimentally seeded stools. The limit of detection of the assay for invasive E. histolytica crude antigen diluted in phosphate-buffered saline or in stools was 0.58 and 3.9 micrograms/ml, respectively, which is the equivalent of approximately 72 and 487 E. histolytica trophozoites per well, respectively. The sensitivity, specificity, and efficiency of the FAC-ELISA were 87, 100, and 98%, respectively, for the detection of invasive E. histolytica antigens and 100, 100, and 100%, respectively, for the diagnosis of amebic dysentery. The FAC-ELISA is a potential alternative for the field diagnosis of amebic dysentery and for epidemiological studies to define the distribution of invasive E. histolytica.
一种针对溶组织内阿米巴的侵袭性菌株特异性单克隆抗体已用于捕获酶联免疫吸附测定（ELISA），以检测临床标本中侵袭性溶组织内阿米巴粪便抗原，并用于诊断来自孟加拉国患者的阿米巴痢疾。粪便抗原捕获ELISA（FAC-ELISA）在临床标本中不与其他寄生虫物种发生交叉反应，也不与这些标本以及实验接种粪便中存在的非侵袭性溶组织内阿米巴发生交叉反应。该测定法对在磷酸盐缓冲盐溶液或粪便中稀释的侵袭性溶组织内阿米巴粗抗原的检测限分别为0.58和3.9微克/毫升，分别相当于每孔约72和487个溶组织内阿米巴滋养体。FAC-ELISA检测侵袭性溶组织内阿米巴抗原的敏感性、特异性和效率分别为87%、100%和98%，诊断阿米巴痢疾分别为100%、100%和100%。FAC-ELISA是阿米巴痢疾现场诊断和确定侵袭性溶组织内阿米巴分布流行病学研究的潜在替代方法。
