Axenic massive cultivation of Entamoeba histolytica trophozoites in spinner flasks and in a microfermentor.
作者信息
Said-Fernández S, Mata-Cárdenas B D, Castro-Garza J, Martínez-Rodríguez H, Navarro-Marmolejo L, Lozano-Garza G
机构信息
División de Biología Celular, Instituto Mexicano del Seguro Social, Monterrey, NL.
出版信息
Arch Med Res. 1992;23(2):57-8.
PMID:1340322
Abstract
A medium, PEHPS, whose main components are extracts of ox liver, and ox and swine pancreas (EHP) was used to develop two alternative methods for axenic cultivation of trophozoites in suspension: with magnetic stirrer and with a 14 l capacity microfermentor, giving the first technic yields of 2.36 x 10(5) to 3.08 x 10(5) and the second in a 14 l capacity microfermentor, which produced 1.65 x 10(5) amebas/ml in one batch and 2.94 to 3.65 x 10(5) of such parasites/ml at semicontinuous flux.
摘要
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