在转瓶和微型发酵罐中对溶组织内阿米巴滋养体进行无菌大规模培养。
Axenic massive cultivation of Entamoeba histolytica trophozoites in spinner flasks and in a microfermentor.
作者信息
Said-Fernández S, Mata-Cárdenas B D, Castro-Garza J, Martínez-Rodríguez H, Navarro-Marmolejo L, Lozano-Garza G
机构信息
División de Biología Celular, Instituto Mexicano del Seguro Social, Monterrey, NL.
出版信息
Arch Med Res. 1992;23(2):57-8.
A medium, PEHPS, whose main components are extracts of ox liver, and ox and swine pancreas (EHP) was used to develop two alternative methods for axenic cultivation of trophozoites in suspension: with magnetic stirrer and with a 14 l capacity microfermentor, giving the first technic yields of 2.36 x 10(5) to 3.08 x 10(5) and the second in a 14 l capacity microfermentor, which produced 1.65 x 10(5) amebas/ml in one batch and 2.94 to 3.65 x 10(5) of such parasites/ml at semicontinuous flux.
一种名为PEHPS的培养基，其主要成分是牛肝提取物以及牛和猪的胰腺（EHP），被用于开发两种在悬浮液中对滋养体进行无菌培养的替代方法：一种是使用磁力搅拌器，另一种是使用容量为14升的微型发酵罐。第一种方法得到的产量为2.36×10⁵至3.08×10⁵，而在容量为14升的微型发酵罐中使用第二种方法，一批可产生1.65×10⁵个变形虫/毫升，在半连续通量下可产生2.94至3.65×10⁵个此类寄生虫/毫升。
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