Murata M, Sasaki M, Yokoyama A, Iwashita T, Gusovsky F, Daly J W, Yasumoto T
Faculty of Agriculture, Tohoku University, Sendai, Japan.
Bull Soc Pathol Exot. 1992;85(5 Pt 2):470-3.
Partial structures of maitotoxin (MTX) were deduced by extensive two-dimensional NMR measurements, showing that the toxin had carbon-carbon double bonds in both termini of the molecule, and a primary alcohol group at the end. These functionalities were used for preparation of a radioligand of MTX; [3H]H-MTX and [3H]benzoyl-MTX. Both radioligands had high levels of non-specific binding to tissues. The binding of [3H]MTX to rat glioma C6 cells was inhibited by a didesulfo-MTX, suggesting the presence of the specific site for MTX-binding on the external surface of the cell membrane.
通过广泛的二维核磁共振测量推断出了刺尾鱼毒素（MTX）的部分结构，结果表明该毒素在分子的两个末端均含有碳 - 碳双键，且末端有一个伯醇基团。这些官能团被用于制备MTX的放射性配体；[3H]H - MTX和[3H]苯甲酰 - MTX。两种放射性配体均与组织有高水平的非特异性结合。[3H]MTX与大鼠胶质瘤C6细胞的结合受到双去硫MTX的抑制，这表明细胞膜外表面存在MTX结合的特定位点。
