Day R W, Lai W W, Klitzner T S, Ignarro L J
University of California, Los Angeles School of Medicine.
Dev Pharmacol Ther. 1992;19(2-3):90-8. doi: 10.1159/000457469.
To determine the influence of oxygen on aortic tension in young and mature animals, we evaluated the response of neonatal and adult rabbit aortic rings to changes in oxygen tension. In 95% nitrogen/5% carbon dioxide, endothelium-derived nitric oxide was not a factor in the development or maintenance of low resting aortic ring tensions. However, the production of endothelium-derived nitric oxide increased in 21% oxygen/74% nitrogen/5% carbon dioxide and 95% oxygen/5% carbon dioxide, and attenuated a concomitant endothelium-independent mechanism of increasing vascular tension. A protein kinase inhibitor, 0.2 mM 1-(5-isoquinolinyl-sulfonyl)-2-methyl piperazine (H-7) inhibited oxygen-mediated constriction of neonatal vessels, but also significantly reduced the contractile response to 60 mM KCl. In conclusion, resting aortic tension is in part determined by oxygen-mediated control of the production of endothelium-derived nitric oxide and one or more opposing endothelium-independent mechanisms of constriction.
为了确定氧气对幼年和成年动物主动脉张力的影响，我们评估了新生兔和成年兔主动脉环对氧张力变化的反应。在95%氮气/5%二氧化碳环境中，内皮衍生的一氧化氮不是低静息主动脉环张力产生或维持的因素。然而，在21%氧气/74%氮气/5%二氧化碳和95%氧气/5%二氧化碳环境中，内皮衍生的一氧化氮生成增加，并减弱了伴随的非内皮依赖性血管张力增加机制。一种蛋白激酶抑制剂，0.2 mM 1-(5-异喹啉磺酰基)-2-甲基哌嗪（H-7）抑制了氧气介导的新生血管收缩，但也显著降低了对60 mM氯化钾的收缩反应。总之，静息主动脉张力部分由氧气介导的内皮衍生一氧化氮生成控制以及一种或多种相反的非内皮依赖性收缩机制决定。
