Ottonello L, Dapino P, Pastorino G, Dallegri F
Department of Internal Medicine, University of Genova Medical School, Italy.
J Clin Lab Immunol. 1992;37(2):91-6.
The superoxide (O2) production by phagocytes (neutrophils plus monocytes) and the lactoferrin release by neutrophils were measured in normal volunteers before and after the oral administration of the anti-inflammatory drug nimesulide. The chemotactic factor N-formylmethionyl-leucyl-phenylalanine (FMLP) and opsonized zymosan particles (OPZ) were used as activating stimuli. The oral administration of nimesulide lowered the phagocyte ability to generate O2- in response to both FMLP (percent inhibition = 67.62) and OPZ (percent inhibition = 36.75). The lactoferrin release by neutrophils was unaffected, proving that the drug does not affect the exocytosis of specific granules. The results provide direct evidence that the oral administration of nimesulide efficiently reduces the oxidative potential of phagocytes, particularly neutrophils, without interfering with mechanisms related to exocytosis of specific granules and involved in the amplification of the cell responses to inflammatory mediators.
在正常志愿者口服抗炎药物尼美舒利前后，检测了吞噬细胞（中性粒细胞加单核细胞）产生超氧化物（O₂）的情况以及中性粒细胞释放乳铁蛋白的情况。趋化因子N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（FMLP）和调理酵母聚糖颗粒（OPZ）用作激活刺激物。口服尼美舒利降低了吞噬细胞对FMLP（抑制百分比 = 67.62）和OPZ（抑制百分比 = 36.75）产生O₂⁻的能力。中性粒细胞释放乳铁蛋白未受影响，证明该药物不影响特定颗粒的胞吐作用。结果提供了直接证据，表明口服尼美舒利可有效降低吞噬细胞，特别是中性粒细胞的氧化潜能，而不会干扰与特定颗粒胞吐作用相关且参与细胞对炎症介质反应放大的机制。
