Ryba M, Walski M, Iwańska K, Głowicki K, Pastuszko M
Department of Neurophysiology, Polish Academy of Sciences, Warsaw.
Neuropatol Pol. 1992;30(3-4):263-70.
Experiments were performed on six male cats under nitrous oxide in oxygen anesthesia. Animals were paralysed and artificially ventilated. Subarachnoid hemorrhage (SAH) was produced by basilar artery puncture following clivectomy. After 10 minutes (3 cats) and 60 minutes (3 cats) the basilar artery was dissected and fixed for electronmicroscopic examination. Ultrastructural examinations revealed in the cytoplasm of the endothelial cells on the side of the vessel lumen, the presence of spherical or flattened cylindrical structures identified as Weibel-Palade bodies. In the endothelium of the proximal segment, 10 and 60 minutes after puncture, the number of these electron-dense bodies increased, and was higher than in the corresponding distal segment. The possible role of the Weibel-Palade bodies in SAH after-effects is discussed.
实验在六只雄性猫身上进行，采用氧化亚氮加氧气麻醉。动物被麻痹并进行人工通气。在颅骨切除术后通过基底动脉穿刺造成蛛网膜下腔出血（SAH）。10分钟后（3只猫）和60分钟后（3只猫），解剖基底动脉并固定用于电子显微镜检查。超微结构检查显示，在血管腔一侧的内皮细胞胞质中，存在被鉴定为魏尔-帕拉德小体的球形或扁平圆柱形结构。在穿刺后10分钟和60分钟的近端节段内皮中，这些电子致密小体的数量增加，且高于相应的远端节段。文中讨论了魏尔-帕拉德小体在SAH后遗症中的可能作用。
