de Carvalho W B, Kopelman B I, de Araújo P S
Department of Pediatrics, Escola Paulista de Medicina, São Paulo, Brasil.
Rev Paul Med. 1992 May-Jun;110(3):138-44.
The present study was undertaken to determine the correlation between free bilirubin and indirect bilirubin in normal newborn infants with non-hemolytic jaundice, and the possible effect of hemolysis on free bilirubin measurement by the peroxidase method. A prospective study protocol was applied at the Neonatal Unit of the Department of Pediatrics, Escola Paulista de Medicina. Forty-three newborn infants were submitted to measurement of free bilirubin and bilirubin fractions and the extent of hemolysis of the sample was determined. Data were analyzed statistically by the Student t-test. A positive and moderate correlation (r = 0.668; p < 0.01) was detected between free bilirubin and indirect bilirubin. The linear regression equation calculated by the least squares method was as follows: f(x) = 4.562 + 0.382x. The concentration of free bilirubin was inversely proportional to sample hemolysis, the difference being greater at 50 mg/dl hemolysis. Despite these results, however, the use of this correlation is delicate due to the impossibility of establishing it in individual cases. Also, since the samples may show some degree of hemolysis, this factor should be minimized by appropriate sample collection before free bilirubin measurement.
本研究旨在确定非溶血性黄疸正常新生儿中游离胆红素与间接胆红素之间的相关性，以及溶血对过氧化物酶法测量游离胆红素的可能影响。在圣保罗医学院儿科学系新生儿科采用了前瞻性研究方案。对43例新生儿进行了游离胆红素和胆红素组分的测量，并测定了样本的溶血程度。数据采用Student t检验进行统计学分析。游离胆红素与间接胆红素之间存在正相关且中等程度的相关性（r = 0.668；p < 0.01）。用最小二乘法计算的线性回归方程如下：f(x) = 4.562 + 0.382x。游离胆红素浓度与样本溶血呈反比，在溶血50mg/dl时差异更大。然而，尽管有这些结果，但由于无法在个别病例中建立这种相关性，因此这种相关性的应用较为棘手。此外，由于样本可能存在一定程度的溶血，在测量游离胆红素之前，应通过适当的样本采集将这一因素降至最低。
