HERRIOTT R M, BARLOW J L
J Gen Physiol. 1957 May 20;40(5):809-25. doi: 10.1085/jgp.40.5.809.
A method of preparing the protein coats or ghosts of phage T2 is described along with proof that the lytic action is a property of the ghost. An assay based on the lytic action toward host cells has been developed which permits a rapid evaluation of the number of ghosts with a reliability of +/-15 per cent. The antigenic and certain physicochemical properties of the ghost have been determined.
本文描述了一种制备噬菌体T2蛋白质外壳或空斑的方法,并证明了裂解作用是空斑的一种特性。已开发出一种基于对宿主细胞裂解作用的测定方法,该方法能够快速评估空斑数量,其可靠性为±15%。已确定了空斑的抗原性和某些物理化学性质。