Binninger I, Clavel C, Polette M, Boutterin M C, Chypre C, Alpha B, Birembaut P
Laboratoire Pol Bouin, I.N.S.E.R.M. U.314, Hôpital Maison Blanche, Reims, France.
Diagn Mol Pathol. 1992 Dec;1(4):239-45.
A novel technique using a two-step polymerase chain reaction (PCR) with specific primers detecting human papillomavirus (HPV) DNA of types 6/11, 16, and 18 and a final nonisotopic colorimetric detection has been developed. Sixty formalin-fixed and paraffin-embedded sections were treated with this methodology and the results compared with those obtained with in situ hybridization (ISH). Twenty cases displaying HPV DNA with ISH were positive with PCR. Seven (35%) of 20 cases negative for ISH but evocative of HPV infection with classic histology displayed HPV DNA with the two-step PCR. Only one case (5%) of 20 normal tissues and/or inflammatory lesions not evocative of HPV infection and negative upon ISH showed HPV DNA. This original technique allows rapid, highly sensitive, and specific detection of HPV DNA and is suitable for most laboratories.
一种采用两步聚合酶链反应（PCR）结合特异性引物检测6/11型、16型和18型人乳头瘤病毒（HPV）DNA，并进行最终非同位素比色检测的新技术已被开发出来。60个福尔马林固定石蜡包埋切片用该方法处理，结果与原位杂交（ISH）所得结果进行比较。ISH显示HPV DNA阳性的20个病例PCR检测也呈阳性。ISH检测为阴性但经典组织学提示HPV感染的20个病例中，有7个（35%）通过两步PCR检测显示HPV DNA阳性。20个正常组织和/或无HPV感染迹象且ISH检测为阴性的炎性病变中，只有1个病例（5%）显示HPV DNA阳性。这项新技术能够快速、高度灵敏且特异检测HPV DNA，适用于大多数实验室。
