del Portillo H A, Levitus G, Camargo L M, Ferreira M U, Mertens F
Departamento de Parasitologia, Universidade de São Paulo, Brasil.
Mem Inst Oswaldo Cruz. 1992;87 Suppl 3:77-84. doi: 10.1590/s0074-02761992000700010.
The complete primary structure of the gene encoding the Merozoite Surface Protein 1 of Plasmodium vivax (PvMSP-1) revealed the existence of interspecies conserved regions among the analogous proteins of other Plasmodia species. Here, three DNA recombinant clones expressing 50, 200 and 500 amino acids from the N-terminal region of the PvMSP-1 protein were used on ELISA and protein immunoblotting assays to look at the IgG antibody responses of malaria patients from the Brazilian amazon region of Rondônia. The results showed the existence of P. vivax and P. falciparum IgG antibodies directed against PvMSP-1 antigenic determinants expressed in the clones containing the first 200 and the following 500 amino acids of the molecule, but not within the one expressing the most N-terminal 50 amino acids. Interestingly, there was no correlation between the levels of these IgG antibodies and the previous number of malaria infections.
间日疟原虫裂殖子表面蛋白1（PvMSP-1）编码基因的完整一级结构显示，在其他疟原虫物种的类似蛋白中存在种间保守区域。在此，使用表达PvMSP-1蛋白N端区域50、200和500个氨基酸的三个DNA重组克隆进行酶联免疫吸附测定（ELISA）和蛋白质免疫印迹分析，以观察来自巴西朗多尼亚州亚马逊地区疟疾患者的IgG抗体反应。结果显示，针对在包含该分子前200个氨基酸及随后500个氨基酸的克隆中表达的PvMSP-1抗原决定簇，存在间日疟原虫和恶性疟原虫的IgG抗体，但在表达最N端50个氨基酸的克隆中未检测到。有趣的是，这些IgG抗体水平与既往疟疾感染次数之间没有相关性。
