Fuenmayor S L, Rodriguez Lemoine V
Centro de Biología Celular, Facultad de Ciencias, Universidad Central de Venezuela, Caracas.
Acta Cient Venez. 1992;43(6):349-54.
Nine Pseudomonas strains, able to degrade polycycle aromatic hydrocarbons (PAHs), were isolated from enriched cultures with naphthalene, as carbon source, and soil samples from a land farming process applied on oil sludge, as inocula. Degradative tests showed that all the strains were capable to catabolize naphthalene (Nah) and phenanthrene (Phn). U2 strain transferred the selected function (Nah) to P. aeruginosa T1 (Hgr Oct+), however some of the transconjugants lost the Oct character, suggesting that it is of plasmidic nature. T1 derivatives as well the wild strains U28 and U31 transferred Nah function to P. putida AC165. All of the examined transconjugants also catabolized phenanthrene, suggesting that Nah and Phn functions in U2, U28, and U31 strains are linked and probably encoded by transferable plasmids.
从以萘为碳源的富集培养物以及用于油泥土地耕作过程的土壤样品（作为接种物）中分离出了九株能够降解多环芳烃（PAHs）的假单胞菌菌株。降解试验表明，所有菌株都能够分解代谢萘（Nah）和菲（Phn）。U2菌株将选定的功能（Nah）转移到了铜绿假单胞菌T1（Hgr Oct +）中，然而一些接合子失去了Oct特性，这表明它具有质粒性质。T1衍生物以及野生菌株U28和U31将Nah功能转移到了恶臭假单胞菌AC165中。所有检测的接合子也都能分解代谢菲，这表明U2、U28和U31菌株中的Nah和Phn功能是相关联的，并且可能由可转移质粒编码。
