果蝇金属硫蛋白启动子在蚊细胞中具有诱导性。

A Drosophila metallothionein promoter is inducible in mosquito cells.

作者信息

Kovach M J, Carlson J O, Beaty B J

机构信息

Department of Microbiology, Colorado State University, Fort Collins 80526.

出版信息

Insect Mol Biol. 1992;1(1):37-43. doi: 10.1111/j.1365-2583.1993.tb00075.x.

DOI:10.1111/j.1365-2583.1993.tb00075.x

PMID:1343774

Abstract

Expression from a Drosophila metallothionein promoter (Mtn) was investigated in mosquito cells. Recombinant plasmids carrying a transcription unit comprised of the Escherichia coli beta-galactosidase gene (lacZ) fused to the metallothionein promoter were stably introduced into Aedes albopictus C6/36 cells. A low copy transformant containing approximately 60 copies of plasmid per cell, and a high copy transformant (1-2 x 10(4) copies/cell) were characterized. The expression of beta-galactosidase from the metallothionein promoter could be induced and controlled in this heterologous system, even when the copy number of introduced plasmid was several thousand.

摘要

在蚊细胞中研究了来自果蝇金属硫蛋白启动子（Mtn）的表达。携带由与金属硫蛋白启动子融合的大肠杆菌β-半乳糖苷酶基因（lacZ）组成的转录单位的重组质粒被稳定地导入白纹伊蚊C6/36细胞。对每个细胞含有约60个质粒拷贝的低拷贝转化体和高拷贝转化体（1 - 2×10⁴个拷贝/细胞）进行了表征。即使导入质粒的拷贝数为数千个，在这个异源系统中，金属硫蛋白启动子驱动的β-半乳糖苷酶的表达也能够被诱导和调控。