一种用于细胞培养中流感病毒的新中和方法。
A new neutralization method for influenza virus in cell culture.
作者信息
Taguchi F, Hashimoto O, Matsuzaki T, Lee S M
机构信息
Department of Microbiology, School of Hygienic Sciences, Kitasato University, Japan.
出版信息
Kitasato Arch Exp Med. 1992 Dec;65(4):181-6.
A novel neutralization method (ELISA-NT) has been developed for simple detection of neutralizing antibodies to influenza virus in which type specific soluble (S) CF antigen produced by unneutralized virus was measured by anti-S immune (probe) serum and enzyme-labelled Clq. Neutralizing antibody activity was determined within 48 h and expressed as a new term, NT% of the quantity of neutralized virus. A good correlation was found between NT% determined by the ELISA-NT method and NT titer by the conventional method in 82 human serum samples (r = 0.941 for type A and r = 0.875 for type B of influenza virus, p < 0.01).
已开发出一种新型中和方法（ELISA-NT），用于简单检测流感病毒中和抗体。该方法通过抗S免疫（探针）血清和酶标记的Clq来测量未中和病毒产生的型特异性可溶性（S）CF抗原。中和抗体活性在48小时内测定，并表示为一个新术语，即中和病毒量的NT%。在82份人血清样本中，ELISA-NT方法测定的NT%与传统方法测定的NT滴度之间存在良好的相关性（甲型流感病毒r = 0.941，乙型流感病毒r = 0.875，p < 0.01）。
Zotero 插件