Sasahara T, Ikewaki N, Tamauchi H, Osawa N
Department of Microbiology, Kitasato University School of Medicine, Kanagawa, Japan.
Kitasato Arch Exp Med. 1992 Dec;65(4):225-37.
Characteristics of peritoneal macrophages recovered from mice infected with two attenuated strains SER and Jena of Salmonella enteritidis were compared. Strong resistance against lethal infection with a virulent strain 116-54 of S. enteritidis was seen in a group of mice immunized with strain SER, but not in a group of mice immunized with strain Jena as well as in a control group. Peritoneal macrophages from mice immunized with strain SER showed an enhanced Salmonella-killing activity, an increased generation of O2- and an increased expression of Ia antigen on 7 to 14 days after infection when compared with those from mice immunized with strain Jena and thioglycollate(TG)-elicited macrophages as a control. The bacterial number of strain Jena in organs decreased more rapidly than that of strain SER after day 4 of infection. These observations suggest that the survival of an attenuated Salmonella bacilli at reticulo-endothelium is essential to increase of their activities of macrophages. Macrophages from mice injected with recombinant interferon(IFN)-gamma for 3 days induced the activated stage of the same characteristics as noted in activated macrophages from mice immunized with strain SER. Effect of oxygen intermediates (OI) scavengers such as superoxide dismutase and catalase on Salmonella-killing activity of activated macrophages was not seen at all. These results suggest that an increased generation of OI may be not primarily responsible for the ability to inhibit the intracellular growth of a virulent strain of S. enteritidis in macrophages activated by immunization with live, attenuated strains and injection with rIFN-gamma.
比较了从感染肠炎沙门氏菌两种减毒株SER和耶拿的小鼠中回收的腹腔巨噬细胞的特征。在用SER株免疫的一组小鼠中，观察到对肠炎沙门氏菌强毒株116 - 54的致死感染具有强大抵抗力，但在用耶拿株免疫的一组小鼠以及对照组中未观察到这种情况。与用耶拿株免疫的小鼠和作为对照的巯基乙酸盐（TG）诱导的巨噬细胞相比，用SER株免疫的小鼠腹腔巨噬细胞在感染后7至14天显示出增强的沙门氏菌杀伤活性、增加的O2-生成以及Ia抗原表达增加。感染第4天后，耶拿株在器官中的细菌数量比SER株下降得更快。这些观察结果表明，减毒沙门氏菌杆菌在网状内皮系统中的存活对于其巨噬细胞活性的增加至关重要。用重组干扰素（IFN）-γ注射3天的小鼠的巨噬细胞诱导出与用SER株免疫的小鼠的活化巨噬细胞相同特征的活化阶段。超氧化物歧化酶和过氧化氢酶等氧中间体（OI）清除剂对活化巨噬细胞的沙门氏菌杀伤活性完全没有影响。这些结果表明，OI生成增加可能不是在用减毒活菌株免疫和注射rIFN-γ激活的巨噬细胞中抑制肠炎沙门氏菌强毒株细胞内生长能力的主要原因。
