Naik S, Santangini H, Gann K, Jauregui H
Department of Pathology, Rhode Island Hospital, Providence 02903.
Cell Transplant. 1992;1(1):61-9. doi: 10.1177/096368979200100110.
Substrates used to immobilize hepatocytes for transplantation govern attachment and long-term metabolic activity of these cells. The choice of these substrates is based on the familiarity with proteinaceous materials that are constituents of the extracellular matrix. The use of substrates that recognize carbohydrates on the cell surface may provide an alternative method to attach adult mammalian hepatocytes. In this study, immobilized lectins on tissue culture plasticware were used to support hepatocyte attachment. Long-term cultures with these substrates were compared with control cultures seeded on a mixture of collagen types I and III (Vitrogen). To evaluate the attachment efficiency and long-term maintenance of diazepam metabolic activity of hepatocytes seeded on different commercially available plasticware, four different types of polymers (supplied as 60-mm dishes) were tested. Diazepam, a benzodiazepine metabolized by the P450 intracytoplasmic pathway, is associated with a synaptic receptor (GABA-benzodiazepine receptor) which plays an important role in hepatic coma. Polymethylpentene, a derivative of polypropylene treated by plasma discharge, was the best polymer to maintain P450 phenotypic expression, although other polymers provided similar cell attachment efficiencies. The amounts of adsorbed concanavalin A, Arachis hypogaea (peanut), Lens culinaris, and Pisum sativum agglutinin correlate with the percentage values of hepatocyte attachment. Cell attachment to wheat germ agglutinin increased with increased lectin concentrations in spite of constant amounts of adsorbed lectin, whereas hepatocyte attachment to Bandieraea simplicifolia agglutinin was lower and did not change at different lectin concentrations.(ABSTRACT TRUNCATED AT 250 WORDS)
用于固定肝细胞以便移植的基质决定了这些细胞的附着以及长期代谢活性。这些基质的选择基于对细胞外基质组成成分中蛋白质材料的了解。使用能够识别细胞表面碳水化合物的基质可能为附着成年哺乳动物肝细胞提供一种替代方法。在本研究中，组织培养塑料制品上固定的凝集素被用于支持肝细胞附着。将使用这些基质的长期培养物与接种在I型和III型胶原蛋白混合物（Vitrogen）上的对照培养物进行比较。为了评估接种在不同市售塑料制品上的肝细胞的附着效率以及地西泮代谢活性的长期维持情况，测试了四种不同类型的聚合物（以60毫米培养皿形式提供）。地西泮是一种通过P450胞质内途径代谢的苯二氮䓬类药物，与一种突触受体（GABA - 苯二氮䓬受体）相关，该受体在肝昏迷中起重要作用。聚甲基戊烯是一种经等离子体放电处理的聚丙烯衍生物，是维持P450表型表达的最佳聚合物，尽管其他聚合物提供了相似的细胞附着效率。吸附的刀豆球蛋白A、花生凝集素、菜豆凝集素和豌豆凝集素的量与肝细胞附着的百分比值相关。尽管吸附的凝集素量恒定，但随着凝集素浓度增加，细胞与麦胚凝集素的附着增加，而肝细胞与单叶蔓荆凝集素的附着较低且在不同凝集素浓度下不变。（摘要截短于250字）
