Király A, Sütö G, Vincze A, Tóth G, Matus Z, Mózsik G
Department of Chemistry, Medical University, Pécs, Hungary.
Acta Physiol Hung. 1992;80(1-4):213-8.
As to earlier observations that beta-carotene prevents the development of gastric mucosal injury produced by different noxious agent, however, its cytoprotective effect can be abolished by acute surgical vagotomy. The aim of this study was to evaluate the possible correlation between the gastric mucosal cytoprotective effect of beta-carotene and its gastric mucosal level in rats treated with IND. The gastric mucosal damage was produced by the administration of IND (20 mg/kg s.c.). The instillation of beta-carotene and acute surgical vagotomy (ASV) or SHAM operation were carried out 30 min before IND treatment. The rats were sacrificed 4 h after IND application, and the number and severity of gastric mucosal erosions were noted. The blood rats was collected quantitatively, the liver and the gastric mucosa were removed, and the beta-carotene and vitamin A level of the gastric mucosa, serum and liver were measured with HPLC. It was found that: 1. Beta-carotene induced gastric cytoprotection in SHAM-operated rats treated with IND but its effect disappeared after ASV. 2. Although the beta-carotene level of the gastric mucosa increased its concentration was not elevated in the serum of intact and vagotomized animals either. 3. Vitamin A Formation was not detected in the liver of animals with or without ASV. It was concluded that the lack of intake of beta-carotene into the gastric mucosa can not play etiologic role in the failure of gastric cytoprotection of rats with acute bilateral surgical vagotomy.
然而，关于早期观察到的β-胡萝卜素可预防由不同有害剂引起的胃黏膜损伤的发展，其细胞保护作用可通过急性手术切断迷走神经而消除。本研究的目的是评估在接受吲哚美辛（IND）治疗的大鼠中，β-胡萝卜素的胃黏膜细胞保护作用与其胃黏膜水平之间可能存在的相关性。胃黏膜损伤是通过给予IND（20mg/kg皮下注射）产生的。在IND治疗前30分钟进行β-胡萝卜素滴注以及急性手术切断迷走神经（ASV）或假手术。在给予IND后4小时处死大鼠，记录胃黏膜糜烂的数量和严重程度。定量采集大鼠血液，取出肝脏和胃黏膜，用高效液相色谱法测定胃黏膜、血清和肝脏中的β-胡萝卜素和维生素A水平。结果发现：1. 在接受IND治疗的假手术大鼠中，β-胡萝卜素诱导胃细胞保护作用，但在ASV后其作用消失。2. 尽管胃黏膜中β-胡萝卜素水平升高，但完整和切断迷走神经动物的血清中其浓度均未升高。3. 在有或没有ASV的动物肝脏中均未检测到维生素A的形成。得出的结论是，胃黏膜中缺乏β-胡萝卜素的摄入在急性双侧手术切断迷走神经的大鼠胃细胞保护失败中不能起到病因学作用。
