Fülöp V, Szigetvári I, Szepesi J, Gáti I
Department of Obstetrics and Gynaecology, Postgraduate Medical School, Budapest, Hungary.
Acta Med Hung. 1992;49(3-4):239-47.
The effect of JEG-3 choriocarcinoma supernatant on human natural killer cell and lymphokine-activated killer cell activity was investigated. Choriocarcinoma supernatants from JEG-3 cell lines were obtained at the time of their optimal growth. K562 erythroblastoid cells were used as target cells for natural killer and lymphokine-activated killer cell mediated lysis in a 51Cr release assay. The choriocarcinoma supernatants had a significant dose-dependent suppressive effect on natural killer and lymphokine-activated killer cell activity. This suppression was more expressed at high effector: target cell ratios. Therefore, choriocarcinoma supernatants appear to have potent inhibitory effects on many aspects of cellular immunity, although, additional studies should be performed to further characterize and identify immunoregulatory molecule(s) in choriocarcinoma supernatants.
研究了JEG-3绒毛膜癌细胞上清液对人自然杀伤细胞和淋巴因子激活的杀伤细胞活性的影响。在JEG-3细胞系生长的最佳时期获取绒毛膜癌细胞上清液。在51Cr释放试验中，将K562成红细胞样细胞用作自然杀伤细胞和淋巴因子激活的杀伤细胞介导裂解的靶细胞。绒毛膜癌细胞上清液对自然杀伤细胞和淋巴因子激活的杀伤细胞活性具有显著的剂量依赖性抑制作用。这种抑制在高效应细胞：靶细胞比例下表现得更为明显。因此，绒毛膜癌细胞上清液似乎对细胞免疫的许多方面具有强大的抑制作用，不过，还应进行更多研究以进一步表征和鉴定绒毛膜癌细胞上清液中的免疫调节分子。
