Peter M G, Dahm K H, Röller H
Z Naturforsch C Biosci. 1976 Mar-Apr;31(3-4):129-31. doi: 10.1515/znc-1976-3-405.
The juvenile hormones (JH) in extracts of Manduca sexta blood were converted to 10-(2,4-dichlorobenzoyloxy)-11-methoxy derivatives and quantitatively determined by gas chromatography with electron capture detection. Early IVth instar larvae contained 0.62 ng JH-I, 1.1ng JH-II and 0.07 ng JH-III per ml blood. Early Vth instar larvae, and adult of both sexes contained the hormones in concentration from 0 to 0.24 ng/ml blood. No hormone was detected in late Vth instar (wandering) larvae. The detection limit in our experiments was less than or equal to 0.003 ng/ml blood.
烟草天蛾血淋巴提取物中的保幼激素（JH）被转化为10 -（2,4 - 二氯苯甲酰氧基）-11 - 甲氧基衍生物，并通过带有电子捕获检测的气相色谱法定量测定。四龄初期幼虫每毫升血淋巴中含有0.62纳克JH - I、1.1纳克JH - II和0.07纳克JH - III。五龄初期幼虫以及雌雄成虫血淋巴中激素浓度为0至0.24纳克/毫升。在五龄末期（游走期）幼虫中未检测到激素。我们实验中的检测限小于或等于0.003纳克/毫升血淋巴。
