Yamakawa K, Nakamura Y
Dept. of Biochemistry, Japanese Foundation for Cancer Research, Tokyo.
Gan To Kagaku Ryoho. 1992 Jan;19(1):1-7.
Recently, remarkable progress in molecular biology has enabled isolation of genes responsible for hereditary tumors such as retinoblastoma (RB), Wilms' tumor (WT), von Recklinghausen neurofibromatosis (NF 1), and familial adenomatous polyposis (FAP). Since patients with FAP develop multiple adenomatous polyps in the colon, some of which progress to colon cancer, isolation of the FAP gene allows us a rare opportunity to study genetic events underlying the well defined morphological changes during progression of colorectal tumors. In this report, we presented an approach called "positional cloning" which has become a powerful tool for identifying genes responsible for hereditary tumors, as well as characteristics of some of such genes.
最近，分子生物学取得了显著进展，使得分离出导致遗传性肿瘤的基因成为可能，这些肿瘤包括视网膜母细胞瘤（RB）、肾母细胞瘤（WT）、冯雷克林霍增氏神经纤维瘤病（NF 1）和家族性腺瘤性息肉病（FAP）。由于FAP患者在结肠中会出现多个腺瘤性息肉，其中一些会发展为结肠癌，因此FAP基因的分离为我们提供了一个难得的机会，来研究结直肠肿瘤进展过程中明确的形态学变化背后的遗传事件。在本报告中，我们介绍了一种称为“定位克隆”的方法，它已成为鉴定遗传性肿瘤相关基因的有力工具，以及其中一些基因的特征。
