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不同的带正电荷氨基酸对大肠杆菌中多跨膜蛋白的拓扑结构有相似的影响。

Different positively charged amino acids have similar effects on the topology of a polytopic transmembrane protein in Escherichia coli.

作者信息

Andersson H, Bakker E, von Heijne G

机构信息

Department of Molecular Biology, Karolinska Institute Center for Biotechnology, NOVUM, Huddinge, Sweden.

出版信息

J Biol Chem. 1992 Jan 25;267(3):1491-5.

PMID:1346135
Abstract

Integral membrane proteins from a wide variety of sources conform to a "positive-inside rule," with many more positively charged amino acids in their cytoplasmic as compared to extracytoplasmic domains. A growing body of experimental work also points to positively charged residues in regions flanking the apolar transmembrane segments as being the main topological determinants. In this paper, we report a systematic comparison of the effects of positively (Arg, Lys, His) as well as negatively (Asp, Glu) charged residues on the membrane topology of a model Escherichia coli inner membrane protein. Our results show that positive charge is indeed the major factor determining the transmembrane topology, with Arg and Lys being of nearly equal efficiency. His, although normally a very weak topological determinant, can be potentiated by a lowering of the cytoplasmic pH. Asp and Glu affect the topology to similar extents and only when present in very high numbers.

摘要

来自多种来源的整合膜蛋白符合“正电荷在内规则”,与胞外结构域相比,其胞质结构域中有更多带正电荷的氨基酸。越来越多的实验工作也表明,非极性跨膜片段两侧区域带正电荷的残基是主要的拓扑决定因素。在本文中,我们报告了对带正电荷(精氨酸、赖氨酸、组氨酸)以及带负电荷(天冬氨酸、谷氨酸)的残基对模型大肠杆菌内膜蛋白膜拓扑结构影响的系统比较。我们的结果表明,正电荷确实是决定跨膜拓扑结构的主要因素,精氨酸和赖氨酸的效率几乎相同。组氨酸虽然通常是一个非常弱的拓扑决定因素,但可以通过降低胞质pH值来增强其作用。天冬氨酸和谷氨酸对拓扑结构的影响程度相似,且只有在数量非常多的时候才会起作用。

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